Validation of Heterophase RNA Analysis with the Use of a Silicon-on-Insulator Biosensor

E. V. Dmitrienko, A. V. Poryvaeva, O. V. Naumova, B. I. Fomin, M. S. Kupryushkin, I. A. Pyshnaya, D. V. Pyshnyi

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

The analysis of nucleic acids remains to be a topical trend in the development of medical diagnostics. Contemporary ultrasensitive diagnostic systems provide the conversion of a specific interaction into a hardwarely-detectable signal in the course of analysis. An example of such diagnostic devices is silicon-on-insulator (SOI) biosensors of field-effect transistors (FET). In this study, glass surfaces are used (as a Si/SiO2 surface for a SOI biosensor) for the optimization and validation of all analysis stages. The efficient immobilization of electroneutral analogs of oligonucleides onto a Si/SiO2surface after the activation of Si-OH groups with 3-glycidoxypropyltrimethoxysilane or carbonyldiimidazole is demonstrated. In the presence of salt at a low concentration or with no salt, the possibility of detecting an RNA target on model glass surfaces in the parallel analysis regime is shown. The regeneration of the Si/SiO2 surface of sensors for repeated analysis and the stability of sensors under long-term storage are also demonstrated.

Язык оригиналаанглийский
Страницы (с-по)44-50
Число страниц7
ЖурналOptoelectronics, Instrumentation and Data Processing
Том57
Номер выпуска1
DOI
СостояниеОпубликовано - янв 2021

Предметные области OECD FOS+WOS

  • 2.02 ЭЛЕКТРОТЕХНИКА, ЭЛЕКТРОННАЯ ТЕХНИКА, ИНФОРМАЦИОННЫЕ ТЕХНОЛОГИИ
  • 1.03 ФИЗИЧЕСКИЕ НАУКИ И АСТРОНОМИЯ

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