Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy

Olesya A. Krumkacheva, Ivan O. Timofeev, Larisa V. Politanskaya, Yuliya F. Polienko, Evgeny V. Tretyakov, Olga Yu Rogozhnikova, Dmitry V. Trukhin, Victor M. Tormyshev, Alexey S. Chubarov, Elena G. Bagryanskaya, Matvey V. Fedin

Результат исследования: Научные публикации в периодических изданияхстатья

4 Цитирования (Scopus)

Аннотация

Precise nanoscale distance measurements by pulsed electron paramagnetic resonance (EPR) spectroscopy play a crucial role in structural studies of biomolecules. The properties of the spin labels used in this approach determine the sensitivity limits, attainable distances, and proximity to biological conditions. Herein, we propose and validate the use of photoexcited fullerenes as spin labels for pulsed dipolar (PD) EPR distance measurements. Hyperpolarization and the narrower spectrum of fullerenes compared to other triplets (e.g., porphyrins) boost the sensitivity, and superior relaxation properties allow PD EPR measurements up to a near-room temperature. This approach is demonstrated using fullerene–nitroxide and fullerene–triarylmethyl pairs, as well as a supramolecular complex of fullerene with nitroxide-labeled protein. Photoexcited triplet fullerenes can be considered as new spin labels with outstanding spectroscopic properties for future structural studies of biomolecules.

Язык оригиналаанглийский
Страницы (с-по)13271-13275
Число страниц5
ЖурналAngewandte Chemie - International Edition
Том58
Номер выпуска38
DOI
СостояниеОпубликовано - 12 авг 2019

Fingerprint Подробные сведения о темах исследования «Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy». Вместе они формируют уникальный семантический отпечаток (fingerprint).

  • Цитировать