Total Correlation Spectroscopy across All NMR-Active Nuclei by Mixing at Zero Field

Ivan V. Zhukov, Alexey S. Kiryutin, Fabien Ferrage, Gerd Buntkowsky, Alexandra V. Yurkovskaya, Konstantin L. Ivanov

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

5 Цитирования (Scopus)


Multidimensional nuclear magnetic resonance (NMR) is based on a combination of well-established building blocks for polarization transfer. These blocks are used to design correlation experiments through one or a few chemical bonds or through space. Here, we introduce a building block that enables polarization transfer across all NMR-active nuclei in a coupled network of spins: isotropic mixing at zero and ultralow field (ZULF). Exploiting mixing under ZULF-NMR conditions, heteronuclear TOtal Correlation SpectroscopY (TOCSY) experiments were developed to highlight coupled spin networks. We demonstrate 1H-13C and 1H-15N correlations in ZULF-TOCSY spectra of labeled amino acids, which allow one to obtain cross-peaks among all heteronuclei belonging to the same coupled network, even when the direct interaction between them is negligible. We also demonstrate the potential of ZULF-TOCSY to analyze complex mixtures on a growth medium of isotope-labeled biomolecules. ZULF-TOCSY enables the quick identification of individual compounds in the mixture by their coupled spin networks. The ZULF-TOCSY method will lead to the development of a new toolbox of experiments to analyze complex mixtures by NMR.

Язык оригиналаанглийский
Страницы (с-по)7291-7296
Число страниц6
ЖурналThe journal of physical chemistry letters
Номер выпуска17
СостояниеОпубликовано - 3 сент. 2020


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