The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication

Tatyana Zykova, Mariya Maltseva, Fedor Goncharov, Lidia Boldyreva, Galina Pokholkova, Tatyana Kolesnikova, Igor Zhimulev

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Although heterochromatin makes up 40% of the Drosophila melanogaster genome, its organization remains little explored, especially in polytene chromosomes, as it is virtually not repre-sented in them due to underreplication. Two all-new approaches were used in this work: (i) with the use of a newly synthesized Drosophila line that carries three mutations, Rif11, SuURES and Su(var)3-906, suppressing the underreplication of heterochromatic regions, we obtained their fullest representation in polytene chromosomes and described their structure; (ii) 20 DNA fragments with known positions on the physical map as well as molecular genetic features of the genome (gene density, histone marks, heterochromatin proteins, origin recognition complex proteins, replication timing sites and satellite DNAs) were mapped in the newly polytenized heterochromatin using FISH and bioinformatics data. The borders of the heterochromatic regions and variations in their positions on arm 3L have been determined for the first time. The newly polytenized heterochro-matic material exhibits two main types of morphology: a banding pattern (locations of genes and short satellites) and reticular chromatin (locations of large blocks of satellite DNA). The locations of the banding and reticular polytene heterochromatin was determined on the physical map.

Язык оригиналаанглийский
Номер статьи2809
ЖурналCells
Том10
Номер выпуска11
DOI
СостояниеОпубликовано - ноя 2021

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