Аннотация
In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.
Язык оригинала | английский |
---|---|
Номер статьи | 190007 |
Страницы (с-по) | 190007 |
Число страниц | 14 |
Журнал | Scientific Data |
Том | 6 |
DOI | |
Состояние | Опубликовано - 5 фев 2019 |
Предметные области OECD FOS+WOS
- 1.02 КОМПЬЮТЕРНЫЕ И ИНФОРМАЦИОННЫЕ НАУКИ
- 1.01 МАТЕМАТИКА
- 5.03 НАУКИ ОБ ОБРАЗОВАНИИ