Temperature dependence of unwinding forces between complementary oligonucleotides

Fedor N. Dultsev, Nina N. Kurus

Результат исследования: Научные публикации в периодических изданияхстатья

1 Цитирования (Scopus)

Аннотация

Rupture Event Scanning (REVS) was used to study oligonucleotide unwinding under mechanical load. Oligonucleotide melting temperature was successfully estimated using this method. To estimate the enthalpy of reaction, we represented denaturation process as a unimolecular reaction. This gave us the possibility to recover the force profile from the experimental data obtained in force measurements at different scanning time (reaction time) for different temperatures.

Язык оригиналаанглийский
Страницы (с-по)94-97
Число страниц4
ЖурналJournal of Microbiological Methods
Том143
DOI
СостояниеОпубликовано - 1 дек 2017

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