Study of calcium signaling dynamics in single platelets using optical activation methods

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференцийстатья в сборнике материалов конференциинаучнаярецензирование

Аннотация

Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.

Язык оригиналаанглийский
Название основной публикацииBiomedical Spectroscopy, Microscopy, and Imaging
РедакторыJurgen Popp, Csilla Gergely
ИздательSPIE
ISBN (электронное издание)9781510634909
DOI
СостояниеОпубликовано - 1 апр 2020
СобытиеBiomedical Spectroscopy, Microscopy, and Imaging 2020 - Virtual, Online, Франция
Продолжительность: 6 апр 202010 апр 2020

Серия публикаций

НазваниеProceedings of SPIE - The International Society for Optical Engineering
Том11359
ISSN (печатное издание)0277-786X
ISSN (электронное издание)1996-756X

Конференция

КонференцияBiomedical Spectroscopy, Microscopy, and Imaging 2020
СтранаФранция
ГородVirtual, Online
Период06.04.202010.04.2020

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