Spectrally encoded microspheres for immunofluorescence analysis

Natalya Sankova, Pavel Shalaev, Viktoriya Semeykina, Sergey Dolgushin, Elena Odintsova, Ekaterina Parkhomchuk

Результат исследования: Научные публикации в периодических изданияхобзорная статьярецензирование

Аннотация

A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis.

Язык оригиналаанглийский
Номер статьи49890
Число страниц21
ЖурналJournal of Applied Polymer Science
Ранняя дата в режиме онлайн28 сен 2020
DOI
СостояниеЭлектронная публикация перед печатью - 28 сен 2020

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