Spectral approach to recognize spherical particles among non-spherical ones by angle-resolved light scattering

Ekaterina S. Yastrebova, Ivan Dolgikh, Konstantin V. Gilev, Irina V. Vakhrusheva, Elizaveta Liz, Alena L. Litvinenko, Vyacheslav M. Nekrasov, Dmitry I. Strokotov, Andrei A. Karpenko, Valeri P. Maltsev

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Most of known light-scattering technologies, which allow one to separate spherical from non-spherical single particles, utilize either analysis of 2D light-scattering pattern or depolarization of light scattered. Both approaches force one to use high-sensitive detectors to provide a suitable signal to noise ratio for two-dimensionless photo matrix or for optical system with crossed polarizers. In this study, we introduce the method for discrimination of spherical and non-spherical single particles. The approach is based on measurement of leading, most intensive, element S11 of light-scattering matrix. To provide maximal signal to noise ratio we specified the light-scattering profile (LSP) in terms of integrated over azimuthal angle S11 as a function of polar scattering angle. The shape-sensitive vector-invariant for individual spherical particles was constructed from the parameters of LSP spectrum. The vector-invariant plays a role of the numerical criterion to identify spherical particles from LSPs. It can be applied to find a sphere with characteristics ranging from 16.5 to 70 and from 0.5 to 7.0 for size and phase-shift parameters respectively (size parameter α = πdn0/λ, where d – sphere diameter, λ – wavelength of the incident light, and n0 – medium refractive index, RI, phase-shift parameter ρ = 2α(m − 1), where relative RI m = n/n0 and n is the sphere RI). These ranges cover all possible characteristics of blood cells within the visible region of wavelengths. The ability of the vector-invariant to recognize spherical cells among non-spherical ones was tested theoretically by LSP databases of optical models of platelets and mature red blood cells. Moreover, experimentally the vector-invariant demonstrated good performance in searching of near-perfect spheres among milk fat globules, isolated nuclei of mononuclear cells, and completely spherized cells in a course of red blood cell lysis.

Язык оригиналаанглийский
Номер статьи106700
ЖурналOptics and Laser Technology
Том135
DOI
СостояниеОпубликовано - мар 2021

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