Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis

Olga Kossinova, Alexey Malygin, Alain Krol, Galina Karpova

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференцийглава/разделнаучнаярецензирование

Аннотация

Chemical approaches are very powerful tools for investigating the molecular structure and architecture of large ribonucleoprotein complexes involving ribosomes and other components of the translation system. Application of RNA nucleotide-specific and cross-linking reagents of a broad specificity range allows the researcher to obtain information on the sites of ligand binding to the ribosome and to each other as well as on the RNA rearrangements caused by the binding. Here, we describe specific chemical approaches including chemical probing and site-directed or bifunctional reagent-mediated cross-linking, which have been used for exploring the mechanism of selenocysteine insertion into a polypeptide chain by mammalian ribosomes.

Язык оригиналаанглийский
Название основной публикацииMethods in Molecular Biology
РедакторыL Chavatte
ИздательHumana Press Inc.
Страницы73-92
Число страниц20
Том1661
ISBN (электронное издание)978-1-4939-7258-6
ISBN (печатное издание)978-1-4939-7257-9
DOI
СостояниеОпубликовано - 1 янв 2018

Серия публикаций

НазваниеMethods in molecular biology (Clifton, N.J.)
ISSN (печатное издание)1064-3745

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  • Цитировать

    Kossinova, O., Malygin, A., Krol, A., & Karpova, G. (2018). Specific chemical approaches for studying mammalian ribosomes complexed with ligands involved in selenoprotein synthesis. В L. Chavatte (Ред.), Methods in Molecular Biology (Том 1661, стр. 73-92). (Methods in molecular biology (Clifton, N.J.)). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7258-6_6