Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo

V. A. Cherenko; E. A. Filipenko; T. S. Golubeva

doi:10.1088/1755-1315/723/2/022041

Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo

V. A. Cherenko, E. A. Filipenko, T. S. Golubeva

Результат исследования: Научные публикации в периодических изданиях › статья по материалам конференции › рецензирование

Аннотация

RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.

Язык оригинала	английский
Номер статьи	022041
Журнал	IOP Conference Series: Earth and Environmental Science
Том	723
Номер выпуска	2
DOI	https://doi.org/10.1088/1755-1315/723/2/022041
Состояние	Опубликовано - 12 апр 2021
Событие	2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021 - Smolensk, Российская Федерация Продолжительность: 25 янв 2021 → …

Предметные области OECD FOS+WOS

1.05 НАУКИ О ЗЕМЛЕ И СМЕЖНЫЕ ЭКОЛОГИЧЕСКИЕ НАУКИ

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10.1088/1755-1315/723/2/022041

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title = "Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo",

abstract = "RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants. ",

author = "Cherenko, {V. A.} and Filipenko, {E. A.} and Golubeva, {T. S.}",

note = "Funding Information: This work is supported by the Russian Science Foundation under grant № 19-74-00067. Publisher Copyright: {\textcopyright} Published under licence by IOP Publishing Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.; 2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021 ; Conference date: 25-01-2021",

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Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo. / Cherenko, V. A.; Filipenko, E. A.; Golubeva, T. S.

В: IOP Conference Series: Earth and Environmental Science, Том 723, № 2, 022041, 12.04.2021.

Результат исследования: Научные публикации в периодических изданиях › статья по материалам конференции › рецензирование

TY - JOUR

T1 - Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo

AU - Cherenko, V. A.

AU - Filipenko, E. A.

AU - Golubeva, T. S.

N1 - Funding Information: This work is supported by the Russian Science Foundation under grant № 19-74-00067. Publisher Copyright: © Published under licence by IOP Publishing Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4/12

Y1 - 2021/4/12

N2 - RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.

AB - RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.

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JO - IOP Conference Series: Earth and Environmental Science

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