Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer

A. N. Shirshova, D. A. Shamovskaya, U. A. Boyarskikh, S. V. Apalko, L. S. Leskov, A. V. Sokolov, S. A. Kovalenko, S. G. Scherbak, I. V. Pikalov, A. E. Kel, M. L. Filipenko

Результат исследования: Научные публикации в периодических изданияхстатья

Аннотация

Colorectal cancer (CRC) is one of the most common cancer types in the world. Timely diagnosis of CRC and adenomatous polyps aided by effective screening techniques can considerably reduce mortality from this disease. MicroRNAs constitute a new class of promising biomarkers for a range of human diseases including cancer. The following article assesses the diagnostic significance of miR-146a concentrations in the blood plasma of patients with colorectal cancer. The main group included patients with stages I to III colorectal cancer (n = 102); the control group comprised patients with chronic colitis, nonspecific ulcerative colitis and Crohn’s disease (n = 58) and healthy individuals (n = 42). MicroRNA levels were quantified by reverse-transcription real-time PCR, revealing significantly higher miR-146a concentrations in the samples of patients with CRC than in the controls (p < 0.0001). The optimal diagnostic sensitivity determined by ROC analysis was 47.3 %, specificity was 91.5 %, with AUC = 0.79 ± 0.018. Our findings demonstrate that the studied approach does not have sufficient specificity, but still suggest that miR-146a can be included into screening tests based on quantification of other microRNAs with improved specificity.

Язык оригиналаанглийский
Страницы (с-по)28-33
Число страниц6
ЖурналBulletin of Russian State Medical University
Том6
Номер выпуска4
DOI
СостояниеОпубликовано - 1 июл 2017

Fingerprint Подробные сведения о темах исследования «Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer». Вместе они формируют уникальный семантический отпечаток (fingerprint).

  • Цитировать

    Shirshova, A. N., Shamovskaya, D. A., Boyarskikh, U. A., Apalko, S. V., Leskov, L. S., Sokolov, A. V., Kovalenko, S. A., Scherbak, S. G., Pikalov, I. V., Kel, A. E., & Filipenko, M. L. (2017). Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer. Bulletin of Russian State Medical University, 6(4), 28-33. https://doi.org/10.24075/brsmu.2017-04-05