Replication timing analysis in polyploid cells reveals rif1 uses multiple mechanisms to promote underreplication in drosophila

Souradip Das, Madison Caballero, Tatyana Kolesnikova, Igor Zhimulev, Amnon Koren, Jared Nordman

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Regulation of DNA replication and copy number is necessary to promote genome stability and maintain cell and tissue function. DNA replication is regulated temporally in a process known as replication timing (RT). Rap1-interacting factor 1 (Rif1) is a key regulator of RT and has a critical function in copy number control in polyploid cells. Previously, we demonstrated that Rif1 functions with SUUR to inhibit replication fork progression and promote underreplication (UR) of specific genomic regions. How Rif1-dependent control of RT factors into its ability to promote UR is unknown. By applying a computational approach to measure RT in Drosophila polyploid cells, we show that SUUR and Rif1 have differential roles in controlling UR and RT. Our findings reveal that Rif1 acts to promote late replication, which is necessary for SUUR-dependent underreplication. Our work provides new insight into the process of UR and its links to RT.

Язык оригиналаанглийский
Номер статьиiyab147
ЖурналGenetics
Том219
Номер выпуска3
DOI
СостояниеОпубликовано - нояб. 2021

Предметные области OECD FOS+WOS

  • 1.06.KM ГЕНЕТИКА И НАСЛЕДСТВЕННОСТЬ

Fingerprint

Подробные сведения о темах исследования «Replication timing analysis in polyploid cells reveals rif1 uses multiple mechanisms to promote underreplication in drosophila». Вместе они формируют уникальный семантический отпечаток (fingerprint).

Цитировать