Raman spectra of deuterated hydrocarbons for labeling applications

Konstantin A. Okotrub, Daria V. Shamaeva, Nikolay V. Surovtsev

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Raman spectroscopy is used for studying the composition of cell lipidome. However, the Raman approach suffers from limited distinguish power for different lipids. In recent years, isotopically labeled molecules are increasingly used to enhance the Raman resolution of similar compounds in biological samples. Here, we present Raman study of fully deuterated stearic acid (stearic-d35), semi-deuterated oleic acid (oleic d-9), arachidonic acid with deuterated methine groups (arachidonic-d8), and their hydrogenated analogs. Polarization properties and temperature dependences of CD stretching modes were investigated to reveal the capability of the isotopically shifted Raman peaks in molecular characterization. Sensitivity of Raman peaks to order–disorder transition corresponding was demonstrated for deuterated methine, methylene, and methyl groups. It was found that the isotopically shifted Raman peaks provide information about the orientation of deuterated Raman tags and their surroundings.

Язык оригиналаанглийский
ЖурналJournal of Raman Spectroscopy
Ранняя дата в режиме онлайн15 ноя 2021
DOI
СостояниеЭлектронная публикация перед печатью - 15 ноя 2021

Предметные области OECD FOS+WOS

  • 2.05 ТЕХНОЛОГИЯ МАТЕРИАЛОВ
  • 1.04 ХИМИЧЕСКИЕ НАУКИ

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