Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

M. L. Perepechaeva; T. A. Seredina; Y. A. Sidorova; E. N. Pivovarova; A. L. Markel; V. V. Lyakhovich; A. Y. Grishanova

doi:10.3967/bes2017.041

Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

M. L. Perepechaeva, T. A. Seredina, Y. A. Sidorova, E. N. Pivovarova, A. L. Markel, V. V. Lyakhovich, A. Y. Grishanova

Кафедра физиологии ФЕН

Результат исследования: Научные публикации в периодических изданиях › статья

3 Цитирования (Scopus)

Аннотация

We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

Язык оригинала	английский
Страницы (с-по)	308-313
Число страниц	6
Журнал	Biomedical and Environmental Sciences
Том	30
Номер выпуска	4
DOI	https://doi.org/10.3967/bes2017.041
Состояние	Опубликовано - 1 апр 2017

Доступ к документу

10.3967/bes2017.041

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@article{4eb25f1855ab425b9d62b79e7fbb8ed3,

title = "Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression",

abstract = "We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).",

keywords = "Animals, Antioxidants/pharmacology, Aryl Hydrocarbon Hydroxylases/genetics, Benzo(a)pyrene/toxicity, Carcinogens/toxicity, Environmental Pollutants/toxicity, Gene Expression Regulation, Male, Mutagens/toxicity, Quercetin/pharmacology, RNA, Messenger/genetics, Rats, Rats, Wistar, RAT-LIVER, PROTEIN, TRANSCRIPTION, INDUCTION, 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN, GENE, ARYL-HYDROCARBON RECEPTOR, AH-RECEPTOR, PROMOTER, BINDING",

author = "Perepechaeva, {M. L.} and Seredina, {T. A.} and Sidorova, {Y. A.} and Pivovarova, {E. N.} and Markel, {A. L.} and Lyakhovich, {V. V.} and Grishanova, {A. Y.}",

year = "2017",

month = apr,

day = "1",

doi = "10.3967/bes2017.041",

language = "English",

volume = "30",

pages = "308--313",

journal = "Biomedical and Environmental Sciences",

issn = "0895-3988",

publisher = "Elsevier",

number = "4",

}

Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression. / Perepechaeva, M. L.; Seredina, T. A.; Sidorova, Y. A.; Pivovarova, E. N.; Markel, A. L.; Lyakhovich, V. V.; Grishanova, A. Y.

В: Biomedical and Environmental Sciences, Том 30, № 4, 01.04.2017, стр. 308-313.

Результат исследования: Научные публикации в периодических изданиях › статья

TY - JOUR

T1 - Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

AU - Perepechaeva, M. L.

AU - Seredina, T. A.

AU - Sidorova, Y. A.

AU - Pivovarova, E. N.

AU - Markel, A. L.

AU - Lyakhovich, V. V.

AU - Grishanova, A. Y.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

AB - We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

KW - Animals

KW - Antioxidants/pharmacology

KW - Aryl Hydrocarbon Hydroxylases/genetics

KW - Benzo(a)pyrene/toxicity

KW - Carcinogens/toxicity

KW - Environmental Pollutants/toxicity

KW - Gene Expression Regulation

KW - Male

KW - Mutagens/toxicity

KW - Quercetin/pharmacology

KW - RNA, Messenger/genetics

KW - Rats

KW - Rats, Wistar

KW - RAT-LIVER

KW - PROTEIN

KW - TRANSCRIPTION

KW - INDUCTION

KW - 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

KW - GENE

KW - ARYL-HYDROCARBON RECEPTOR

KW - AH-RECEPTOR

KW - PROMOTER

KW - BINDING

UR - http://www.scopus.com/inward/record.url?scp=85019497129&partnerID=8YFLogxK

U2 - 10.3967/bes2017.041

DO - 10.3967/bes2017.041

M3 - Article

C2 - 28494841

AN - SCOPUS:85019497129

VL - 30

SP - 308

EP - 313

JO - Biomedical and Environmental Sciences

JF - Biomedical and Environmental Sciences

SN - 0895-3988

IS - 4

ER -