TY - JOUR
T1 - Pro-social and anxiolytic-like behavior following a single 24-h exposure to 17β-estradiol in adult male zebrafish
AU - Moraes, Andréia B.
AU - Giacomini, Ana C.V.V.
AU - Genario, Rafael
AU - Marcon, Leticia
AU - Scolari, Naiara
AU - Bueno, Barbara W.
AU - Demin, Konstantin A.
AU - Amstislavskaya, Tamara G.
AU - Strekalova, Tatyana
AU - Soares, Marta C.
AU - de Abreu, Murilo S.
AU - Kalueff, Allan V.
N1 - Funding Information:
ACVVG is supported by the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) research fellowships 17/2551-0001-269-0 . AVK is supported by the Zebrafish Platform Construction Fund from the Southwest University (Chongqing, China) . He chairs the International Zebrafish Neuroscience Research Consortium (ZNRC) that coordinated this multi-laboratory project. The collaboration was supported by the Russian Science Foundation (RSF) grant 19-15-00053 . KAD is supported by the President of Russia Graduate Fellowship , and the Special Rector’s Fellowship for St. Petersburg State University students. TGA is supported by the budgetary state funding for basic research (AAAA-A16-116021010228-0) from the Scientific Research Institute of Neurosciences and Medicine (Novosibirsk, Russia) . The authors thank Sirlei Cazarotto (University of Passo Fundo, Brazil) for her assistance with experimental procedures, and Prof. Denis B. Rosemberg and Luiz V. C. da Rosa (Federal University of Santa Maria, Brazil) for advice and assistance with analyzing shoaling data. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3/16
Y1 - 2021/3/16
N2 - Estradiol (17β-estradiol, E2) is a crucial estrogen hormone that regulates sexual, cognitive, social and affective behaviors in various species. However, complex central nervous system (CNS) effects of E2, including its activity in males, remain poorly understood. The zebrafish (Danio rerio) is rapidly becoming a powerful novel model system in translational neuroscience research. Here, we evaluate the effects of a single 24-h exposure to 20 μg/L of E2 on behavioral and endocrine (cortisol) responses in adult male zebrafish. Overall, E2 exerted pro-social effect in the social preference test, reduced whole-body cortisol levels, elevated exploration in the novel tank test and increased the shoal size in the shoaling test, indicative of an anxiolytic-like profile of this hormone in male zebrafish. Supporting mounting human and rodent evidence on the role of E2 in behavioral regulation, the observed pro-social and anxiolytic-like effects of E2 in male zebrafish reinforce the use of this aquatic organism in studying steroid-mediated CNS mechanisms of complex affective and social behaviors.
AB - Estradiol (17β-estradiol, E2) is a crucial estrogen hormone that regulates sexual, cognitive, social and affective behaviors in various species. However, complex central nervous system (CNS) effects of E2, including its activity in males, remain poorly understood. The zebrafish (Danio rerio) is rapidly becoming a powerful novel model system in translational neuroscience research. Here, we evaluate the effects of a single 24-h exposure to 20 μg/L of E2 on behavioral and endocrine (cortisol) responses in adult male zebrafish. Overall, E2 exerted pro-social effect in the social preference test, reduced whole-body cortisol levels, elevated exploration in the novel tank test and increased the shoal size in the shoaling test, indicative of an anxiolytic-like profile of this hormone in male zebrafish. Supporting mounting human and rodent evidence on the role of E2 in behavioral regulation, the observed pro-social and anxiolytic-like effects of E2 in male zebrafish reinforce the use of this aquatic organism in studying steroid-mediated CNS mechanisms of complex affective and social behaviors.
KW - Anxiety
KW - Anxiety-like behavior
KW - Cortisol
KW - Estrogens
KW - Social behavior
UR - http://www.scopus.com/inward/record.url?scp=85101851809&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2020.135591
DO - 10.1016/j.neulet.2020.135591
M3 - Article
C2 - 33359732
AN - SCOPUS:85101851809
VL - 747
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
M1 - 135591
ER -