Photoreactive DNA as a Tool to Study Replication Protein A Functioning in DNA Replication and Repair

Nadejda I. Rechkunova, Olga I. Lavrik

Результат исследования: Научные публикации в периодических изданияхобзорная статьярецензирование

3 Цитирования (Scopus)


Replication protein A (RPA), eukaryotic single-stranded DNA-binding protein, is a key player in multiple processes of DNA metabolism including DNA replication, recombination and DNA repair. Human RPA composed of subunits of 70-, 32- and 14-kDa binds ssDNA with high affinity and interacts specifically with multiple proteins. The RPA heterotrimer binds ssDNA in several modes, with occlusion lengths of 8–10, 13–22 and 30 nucleotides corresponding to global, transitional and elongated conformations of protein. Varying the structure of photoreactive DNA, the intermediates of different stages of DNA replication or DNA repair were designed and applied to identify positioning of the RPA subunits on the specific DNA structures. Using this approach, RPA interactions with various types of DNA structures attributed to replication and DNA repair intermediates were examined. This review is dedicated to blessed memory of Prof. Alain Favre who contributed to the development of photoreactive nucleotide derivatives and their application for the study of protein–nucleic acids interactions.

Язык оригиналаанглийский
Страницы (с-по)440-449
Число страниц10
ЖурналPhotochemistry and Photobiology
Номер выпуска2
Ранняя дата в режиме онлайн3 фев 2020
СостояниеОпубликовано - 1 мар 2020


Подробные сведения о темах исследования «Photoreactive DNA as a Tool to Study Replication Protein A Functioning in DNA Replication and Repair». Вместе они формируют уникальный семантический отпечаток (fingerprint).