Novel mouse monoclonal antibodies specifically recognize Aspergillus fumigatus galactomannan

Andrey L. Matveev, Vadim B. Krylov, Ljudmila A. Emelyanova, Arsenii S. Solovev, Yana A. Khlusevich, Ivan K. Baykov, Thierry Fontaine, Jean Paul Latgé, Nina V. Tikunova, Nikolay E. Nifantiev

Результат исследования: Научные публикации в периодических изданияхстатья

11 Цитирования (Scopus)

Аннотация

A panel of specific monoclonal antibodies (mAbs) against synthetic pentasaccharide β-D-Galf-(1!5)-[β-D-Galf-(1!5)]3-α-D-Manp, structurally related to Aspergillus fumigatus galactomannan, was generated using mice immunized with synthetic pentasaccharide-BSA conjugate and by hybridoma technology. Two selected mAbs, 7B8 and 8G4, could bind with the initial pentasaccharide with affinity constants of approximately 5.3 nM and 6.4 nM, respectively, based on surface plasmon resonance-based biosensor assay. The glycoarray, built from a series of synthetic oligosaccharide derivatives representing different galactomannan fragments, demonstrated that mAb 8G4 could effectively recognize the parental pentasaccharide while mAb 7B8 recognizes its constituting trisaccharide parts. Immunofluorescence studies showed that both 7B8 and 8G4 could stain A. fumigatus cells in culture efficiently, but not the mutant strain lacking galactomannan. In addition, confocal microscopy demonstrated that Candida albicans, Bifidobacterium longum, Lactobacillus plantarum, and numerous gram-positive and gram-negative bacteria were not labeled by mAbs 7B8 and 8G4. The generated mAbs can be considered promising for the development of a new specific enzyme-linked assay for detection of A. fumigatus, which is highly demanded for medical and environmental controls.

Язык оригиналаанглийский
Номер статьи0193938
Страницы (с-по)e0193938
Число страниц15
ЖурналPLoS ONE
Том13
Номер выпуска3
DOI
СостояниеОпубликовано - 8 мар 2018

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  • Цитировать

    Matveev, A. L., Krylov, V. B., Emelyanova, L. A., Solovev, A. S., Khlusevich, Y. A., Baykov, I. K., Fontaine, T., Latgé, J. P., Tikunova, N. V., & Nifantiev, N. E. (2018). Novel mouse monoclonal antibodies specifically recognize Aspergillus fumigatus galactomannan. PLoS ONE, 13(3), e0193938. [0193938]. https://doi.org/10.1371/journal.pone.0193938