Novel Convenient Approach to the Solid-Phase Synthesis of Oligonucleotide Conjugates

Mariya I. Meschaninova, Darya S. Novopashina, Olga A. Semikolenova, Vladimir N. Silnikov, Alya G. Venyaminova

Результат исследования: Научные публикации в периодических изданияхстатья

Аннотация

A novel and convenient approach for the solid-phase 5'-functionalization of oligonucleotides is proposed in this article. The approach is based on the activation of free 5'-hydroxyl of polymer support-bound protected oligonucleotides by N,N'-disuccinimidyl carbonate followed by interaction with amino-containing ligands. Novel amino-containing derivatives of closo-dodecaborate, estrone, cholesterol, and α-tocopherol were specially prepared. A wide range of oligonucleotide conjugates bearing closo-dodecaborate, short peptide, pyrene, lipophilic residues (cholesterol, α-tocopherol, folate, estrone), aliphatic diamines, and propargylamine were synthesized and characterized to demonstrate the versatility of the approach. The developed method is suitable for the conjugate synthesis of oligonucleotides of different types (ribo-, deoxyribo-, 2'-O-methylribo-, and others).

Язык оригиналаанглийский
Номер статьи4266
Число страниц14
ЖурналMolecules (Basel, Switzerland)
Том24
Номер выпуска23
DOI
СостояниеОпубликовано - 22 ноя 2019

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