TY - JOUR
T1 - Novel cancer stem marker and its applicability for grading primary human gliomas
AU - Dolgova, Evgeniya V.
AU - Mishinov, Sergey V.
AU - Proskurina, Anastasiya S.
AU - Potter, Ekaterina A.
AU - Efremov, Yaroslav R.
AU - Bayborodin, Sergey I.
AU - Tyrinova, Tamara V.
AU - Stupak, Vyacheslav V.
AU - Ostatin, Alexandr A.
AU - Chernykh, Elena R.
AU - Bogachev, Sergey S.
N1 - Publisher Copyright:
© The Author(s) 2018.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability to natively internalize fragmented double-stranded DNA. Using this feature as a marker, we show that 0.1% to 6% of human glioblastoma cells from the bioptates can effectively internalize a fluorescently labeled DNA probe. Of these, using samples from 3 patients, 66% to 100% cells are also positive for CD133, a well-established surface marker of tumor-initiating glioma stem cells. Using the samples from primary malignant brain lesions (33 patients), we demonstrate that tumor grading significantly correlates (R = .71) with the percentage of DNA-internalizing cells. No such correlation is observed for relapse samples (18 patients).
AB - Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability to natively internalize fragmented double-stranded DNA. Using this feature as a marker, we show that 0.1% to 6% of human glioblastoma cells from the bioptates can effectively internalize a fluorescently labeled DNA probe. Of these, using samples from 3 patients, 66% to 100% cells are also positive for CD133, a well-established surface marker of tumor-initiating glioma stem cells. Using the samples from primary malignant brain lesions (33 patients), we demonstrate that tumor grading significantly correlates (R = .71) with the percentage of DNA-internalizing cells. No such correlation is observed for relapse samples (18 patients).
KW - CD133
KW - Glioblastoma
KW - Neurosphere
KW - TAMRA-DNA
KW - Tumor-initiating stem cells
KW - Humans
KW - Neoplastic Stem Cells/metabolism
KW - Brain Neoplasms/metabolism
KW - AC133 Antigen
KW - Biomarkers, Tumor
KW - DNA, Neoplasm/genetics
KW - Neoplasm Grading
KW - Fluorescent Antibody Technique
KW - Glioma/genetics
KW - Tumor Cells, Cultured
UR - http://www.scopus.com/inward/record.url?scp=85056464567&partnerID=8YFLogxK
U2 - 10.1177/1533034617753812
DO - 10.1177/1533034617753812
M3 - Article
C2 - 29375020
AN - SCOPUS:85056464567
VL - 17
SP - 1
EP - 8
JO - Technology in Cancer Research and Treatment
JF - Technology in Cancer Research and Treatment
SN - 1533-0346
ER -