New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection

O. A. Semikolenova, V. M. Golyshev, B. H. Kim, Aliya G. Venyaminova, D. S. Novopashina

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the PyA-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdAPyAdAdA) or the (2'-O-methylribo)adenosine (AmAm PyAAmAm) cluster with those of (dAdAPyAdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system.

Язык оригиналаанглийский
Страницы (с-по)432-440
Число страниц9
ЖурналRussian Journal of Bioorganic Chemistry
Том47
Номер выпуска2
DOI
СостояниеОпубликовано - мар 2021

Предметные области OECD FOS+WOS

  • 1.06 БИОЛОГИЧЕСКИЕ НАУКИ
  • 1.04 ХИМИЧЕСКИЕ НАУКИ

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