New model systems for RNA interference in plants to develop eco-friendly and effective antipathogenic tools

A. V. Urin, T. S. Golubeva

Результат исследования: Научные публикации в периодических изданияхстатья по материалам конференциирецензирование

Аннотация

The methods which are used to combat pathogens of crops, in particular, chemical treatment, pose a threat to environmental safety. RNA interference could be one of the best ways to selectively control pests, but there are not enough methods for introducing dsRNA into plants without creating GMOs. The development of new methods of dsRNA delivery requires affordable and fast gene model systems, the purpose of which is our work. We selected the genes of several carbonic anhydrases functioning in the thylakoids and mitochondria of the whole plant, the suppression of which will be seen phenotypically based on the data on mutants for these genes. Specific primers have been designed, and a PCR reaction has also been carried out to obtain the product of the corresponding mRNA, which will be delivered to the plant in the future using the VIGS system.

Язык оригиналаанглийский
Номер статьи022040
ЖурналIOP Conference Series: Earth and Environmental Science
Том723
Номер выпуска2
DOI
СостояниеОпубликовано - 12 апр 2021
Событие2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021 - Smolensk, Российская Федерация
Продолжительность: 25 янв 2021 → …

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