The methods which are used to combat pathogens of crops, in particular, chemical treatment, pose a threat to environmental safety. RNA interference could be one of the best ways to selectively control pests, but there are not enough methods for introducing dsRNA into plants without creating GMOs. The development of new methods of dsRNA delivery requires affordable and fast gene model systems, the purpose of which is our work. We selected the genes of several carbonic anhydrases functioning in the thylakoids and mitochondria of the whole plant, the suppression of which will be seen phenotypically based on the data on mutants for these genes. Specific primers have been designed, and a PCR reaction has also been carried out to obtain the product of the corresponding mRNA, which will be delivered to the plant in the future using the VIGS system.
|Журнал||IOP Conference Series: Earth and Environmental Science|
|Состояние||Опубликовано - 12 апр 2021|
|Событие||2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021 - Smolensk, Российская Федерация|
Продолжительность: 25 янв 2021 → …
Предметные области OECD FOS+WOS
- 1.05 НАУКИ О ЗЕМЛЕ И СМЕЖНЫЕ ЭКОЛОГИЧЕСКИЕ НАУКИ