New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis

Tatyana Karamysheva, Svetlana Romanenko, Alexey Makunin, Marija Rajičić, Alexey Bogdanov, Vladimir Trifonov, Jelena Blagojević, Mladen Vujošević, Konstantin Orishchenko, Nikolay Rubtsov

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.

Язык оригиналаанглийский
Номер статьи1819
ЖурналCells
Том10
Номер выпуска7
DOI
СостояниеОпубликовано - 19 июл 2021

Предметные области OECD FOS+WOS

  • 3.02 КЛИНИЧЕСКАЯ МЕДИЦИНА
  • 1.06.DR КЛЕТОЧНАЯ БИОЛОГИЯ

Fingerprint

Подробные сведения о темах исследования «New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis». Вместе они формируют уникальный семантический отпечаток (fingerprint).

Цитировать