Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line

Дарья Вячеславовна Ким; Лилия Михайловна Кулишова; Наталья Александровна Торгашева; Дмитрий Олегович Жарков

Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line

Дарья Вячеславовна Ким, Лилия Михайловна Кулишова, Наталья Александровна Торгашева, Дмитрий Олегович Жарков

Результат исследования: Материалы конференций › тезисы › рецензирование

3 Загрузки (Pure)

Аннотация

A well-known approach to investigate protein functions is to generate knockout animals or cell lines. In the case of many base excision repair (BER) proteins (APEX1, POLB, LIG3, XRCC1) these knockout mice die at the early stages of embryonic development. The CRISPR/Cas9 genome editing technology offers a new way to generate knockouts without causing profound gene disruption. Using this technology, we have established human cell lines deficient in the major apurinic/apyrimidinic (AP) endonuclease, APEX1.

Язык оригинала	английский
Страницы	92
Число страниц	1
Состояние	Опубликовано - авг. 2021
Событие	GENOME ENGINEERING: CRISPR FRONTIERS. Virtual meeting - Продолжительность: 18 авг. 2021 → 20 авг. 2021

Конференция

Конференция	GENOME ENGINEERING: CRISPR FRONTIERS. Virtual meeting
Период	18.08.2021 → 20.08.2021

Предметные области OECD FOS+WOS

1.06 БИОЛОГИЧЕСКИЕ НАУКИ

Доступ к документу

CRISPR_Front-2021-000092

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Ким, Дарья Вячеславовна ; Кулишова, Лилия Михайловна ; Торгашева, Наталья Александровна и др. / Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line. Выдержка из GENOME ENGINEERING: CRISPR FRONTIERS. Virtual meeting.1 стр.

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title = "Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line",

abstract = "A well-known approach to investigate protein functions is to generate knockout animals or cell lines. In the case of many base excision repair (BER) proteins (APEX1, POLB, LIG3, XRCC1) these knockout mice die at the early stages of embryonic development. The CRISPR/Cas9 genome editing technology offers a new way to generate knockouts without causing profound gene disruption. Using this technology, we have established human cell lines deficient in the major apurinic/apyrimidinic (AP) endonuclease, APEX1.",

author = "Ким, {Дарья Вячеславовна} and Кулишова, {Лилия Михайловна} and Торгашева, {Наталья Александровна} and Жарков, {Дмитрий Олегович}",

note = "This research was supported by RFBR (grant 20-34-90092).; null ; Conference date: 18-08-2021 Through 20-08-2021",

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Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line. / Ким, Дарья Вячеславовна; Кулишова, Лилия Михайловна; Торгашева, Наталья Александровна и др.

2021. 92 Выдержка из GENOME ENGINEERING: CRISPR FRONTIERS. Virtual meeting.

Результат исследования: Материалы конференций › тезисы › рецензирование

TY - CONF

T1 - Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line

AU - Ким, Дарья Вячеславовна

AU - Кулишова, Лилия Михайловна

AU - Торгашева, Наталья Александровна

AU - Жарков, Дмитрий Олегович

N1 - This research was supported by RFBR (grant 20-34-90092).

PY - 2021/8

Y1 - 2021/8

N2 - A well-known approach to investigate protein functions is to generate knockout animals or cell lines. In the case of many base excision repair (BER) proteins (APEX1, POLB, LIG3, XRCC1) these knockout mice die at the early stages of embryonic development. The CRISPR/Cas9 genome editing technology offers a new way to generate knockouts without causing profound gene disruption. Using this technology, we have established human cell lines deficient in the major apurinic/apyrimidinic (AP) endonuclease, APEX1.

AB - A well-known approach to investigate protein functions is to generate knockout animals or cell lines. In the case of many base excision repair (BER) proteins (APEX1, POLB, LIG3, XRCC1) these knockout mice die at the early stages of embryonic development. The CRISPR/Cas9 genome editing technology offers a new way to generate knockouts without causing profound gene disruption. Using this technology, we have established human cell lines deficient in the major apurinic/apyrimidinic (AP) endonuclease, APEX1.

M3 - Abstract

SP - 92

Y2 - 18 August 2021 through 20 August 2021

ER -