Methods for Molecular Evolution of Polymerases

S. A. Zhukov, A. A. Fokina, S. V. Vasilyeva, D. A. Stetsenko

Результат исследования: Научные публикации в периодических изданияхстатья

Аннотация

In 2018, three scientists shared the Nobel Prize in Chemistry: Frances H. Arnold, “for the directed evolution of enzymes”; George P. Smith and Sir Gregory P. Winter, “for the phage display of peptides and antibodies”. All authors are associated with the development of methods for obtaining the useful proteins and peptides, which are based on imitation of the natural “method” of biological evolution, namely, on a combination of random variability and nonrandom selection. In this review, we consider modern methods for designing engineering polymerases, which determine the progress in the enzymatic synthesis and evolution of unnatural nucleic acids. The development of these methods provides an opportunity to obtain and study a huge set of new biopolymers inaccessible to nature, as well as various ligands, catalysts, and materials based thereon.

Язык оригиналаанглийский
Страницы (с-по)726-738
Число страниц13
ЖурналRussian Journal of Bioorganic Chemistry
Том45
Номер выпуска6
DOI
СостояниеОпубликовано - 1 ноя 2019

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