Аннотация
The design of modified oligonucleotides that combine in one molecule several therapeutically beneficial properties still poses a major challenge. Recently a new type of modified mesyl phosphoramidate (or μ-) oligonucleotide was described that demonstrates high affinity to RNA, exceptional nuclease resistance, efficient recruitment of RNase H, and potent inhibition of key carcinogenesis processes in vitro. Herein, using a xenograft mouse tumor model, it was demonstrated that microRNA miR-21-targeted μ-oligonucleotides administered in complex with folate-containing liposomes dramatically inhibit primary tumor growth via long-term down-regulation of miR-21 in tumors and increase in biosynthesis of miR-21-regulated tumor suppressor proteins. This antitumoral effect is superior to the effect of the corresponding phosphorothioate. Peritumoral administration of μ-oligonucleotide results in its rapid distribution and efficient accumulation in the tumor. Blood biochemistry and morphometric studies of internal organs revealed no pronounced toxicity of μ-oligonucleotides. This new oligonucleotide class provides a powerful tool for antisense technology.
Язык оригинала | английский |
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Страницы (с-по) | 32370-32379 |
Число страниц | 10 |
Журнал | Proceedings of the National Academy of Sciences of the United States of America |
Том | 117 |
Номер выпуска | 51 |
DOI | |
Состояние | Опубликовано - 22 дек. 2020 |
Предметные области OECD FOS+WOS
- 1.07 ПРОЧИЕ ЕСТЕСТВЕННЫЕ И ТОЧНЫЕ НАУКИ