Key aspects of nucleic acid library design for in vitro selection

Maria A. Vorobyeva, Anna S. Davydova, Pavel E. Vorobjev, Dmitrii V. Pyshnyi, Alya G. Venyaminova

Результат исследования: Научные публикации в периодических изданияхобзорная статья

16 Цитирования (Scopus)

Аннотация

Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been established as powerful and tunable tools for biospecific applications, be it therapeutics, drug delivery systems or biosensors. It is now generally acknowledged that in vitro selection enables one to generate aptamers to almost any target of interest. However, the success of selection and the affinity of the resulting aptamers depend to a large extent on the nature and design of an initial random nucleic acid library. In this review, we summarize and discuss the most important features of the design of nucleic acid libraries for in vitro selection such as the nature of the library (DNA, RNA or modified nucleotides), the length of a randomized region and the presence of fixed sequences. We also compare and contrast different randomization strategies and consider computer methods of library design and some other aspects.

Язык оригиналаанглийский
Номер статьи470
Число страниц21
ЖурналInternational Journal of Molecular Sciences
Том19
Номер выпуска2
DOI
СостояниеОпубликовано - 5 фев 2018

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