Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich; N. V. Yunusova; M. N. Stakheeva; A. K. Somov; A. E. Frolova; N. A. Kiryushina; S. G. Afanasyev; A. E. Grigor’eva; P. P. Laktionov; I. V. Kondakova

doi:10.1134/S1990750817030106

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich, N. V. Yunusova, M. N. Stakheeva, A. K. Somov, A. E. Frolova, N. A. Kiryushina, S. G. Afanasyev, A. E. Grigor’eva, P. P. Laktionov, I. V. Kondakova

Кафедра молекулярной биологии и биотехнологии

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

5 Цитирования (Scopus)

Аннотация

A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.

Язык оригинала	английский
Страницы (с-по)	291-295
Число страниц	5
Журнал	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Том	11
Номер выпуска	3
DOI	https://doi.org/10.1134/S1990750817030106
Состояние	Опубликовано - 1 июл 2017

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10.1134/S1990750817030106

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Tamkovich, S. N., Yunusova, N. V., Stakheeva, M. N., Somov, A. K., Frolova, A. E., Kiryushina, N. A., Afanasyev, S. G., Grigor’eva, A. E., Laktionov, P. P., & Kondakova, I. V. (2017). Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 11(3), 291-295. https://doi.org/10.1134/S1990750817030106

Tamkovich, S. N. ; Yunusova, N. V. ; Stakheeva, M. N. ; Somov, A. K. ; Frolova, A. E. ; Kiryushina, N. A. ; Afanasyev, S. G. ; Grigor’eva, A. E. ; Laktionov, P. P. ; Kondakova, I. V. / Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. В: Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 2017 ; Том 11, № 3. стр. 291-295.

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title = "Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients",

abstract = "A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.",

keywords = "breast cancer, colorectal cancer, exosomes, flow cytometry, transmission electron microscopy",

author = "Tamkovich, {S. N.} and Yunusova, {N. V.} and Stakheeva, {M. N.} and Somov, {A. K.} and Frolova, {A. E.} and Kiryushina, {N. A.} and Afanasyev, {S. G.} and Grigor{\textquoteright}eva, {A. E.} and Laktionov, {P. P.} and Kondakova, {I. V.}",

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doi = "10.1134/S1990750817030106",

language = "English",

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Tamkovich, SN, Yunusova, NV, Stakheeva, MN, Somov, AK, Frolova, AE, Kiryushina, NA, Afanasyev, SG, Grigor’eva, AE, Laktionov, PP & Kondakova, IV 2017, 'Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients', Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, том. 11, № 3, стр. 291-295. https://doi.org/10.1134/S1990750817030106

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. / Tamkovich, S. N.; Yunusova, N. V.; Stakheeva, M. N.; Somov, A. K.; Frolova, A. E.; Kiryushina, N. A.; Afanasyev, S. G.; Grigor’eva, A. E.; Laktionov, P. P.; Kondakova, I. V.

В: Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, Том 11, № 3, 01.07.2017, стр. 291-295.

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

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T1 - Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

AU - Tamkovich, S. N.

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AU - Stakheeva, M. N.

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AU - Frolova, A. E.

AU - Kiryushina, N. A.

AU - Afanasyev, S. G.

AU - Grigor’eva, A. E.

AU - Laktionov, P. P.

AU - Kondakova, I. V.

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KW - flow cytometry

KW - transmission electron microscopy

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