Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer

S. N. Tamkovich, A. K. Somov, K. V. Karpukhina, A. E. Grigor'Eva, N. V. Yunusova, M. N. Stakheeva, V. E. Voytsitskiy, I. V. Kondakova, P. P. Laktionov

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференцийстатья в сборнике материалов конференциинаучнаярецензирование

Аннотация

The exosomes containing tumor-specific protein represent a valuable source of material for the non-invasive diagnosis of malignant neoplasms. The exosomes from plasma and cell-associated exosomes from blood of healthy women, the patients with mastopathy and breast cancer patients were studied with the nanoparticle tracking analysis, transmission electron microscopy, flow cytometry and protein profiling. The exposure of CD63, CD24, CD9, and CD81 demonstrates isolation of mainly exosomes. A major part of exosomes in the blood of healthy women, patients with mastopathy and breast cancer patients is shown to be associated with the surface of blood cells. The concentration of the exosomes in the plasma of the breast cancer patients is higher than in the healthy women's plasma. The proteins ranged from 10 to 250 kDa were found in the exosomes by gradient SDS PAAGE; 8 regions of electrophoregram differ between healthy and illness patients in the expression level and number of proteins. These proteins are planned to be identified by MALDI TOF and studied as potential breast cancer markers.

Язык оригиналаанглийский
Название основной публикацииPHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA)
Подзаголовок основной публикацииInterdisciplinary Problems and Clinical Applications - Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017
РедакторыEY Gutmanas, OB Naimark, YP Sharkeev
ИздательAmerican Institute of Physics Inc.
Число страниц5
Том1882
ISBN (электронное издание)9780735415621
DOI
СостояниеОпубликовано - 28 сен 2017
СобытиеInternational Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 - Tomsk, Российская Федерация
Продолжительность: 23 мая 201726 мая 2017

Серия публикаций

НазваниеAIP Conference Proceedings
ИздательAMER INST PHYSICS
Том1882
ISSN (печатное издание)0094-243X

Конференция

КонференцияInternational Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017
СтранаРоссийская Федерация
ГородTomsk
Период23.05.201726.05.2017

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