High-pressure liquid chromatography with direct injection of gas sample

Anton I. Astanin, Grigory I. Baram

Результат исследования: Научные публикации в периодических изданияхстатья

Аннотация

The conventional method of using liquid chromatography to determine the composition of a gaseous mixture entails dissolving vapors in a suitable solvent, then obtaining a chromatograph of the resulting solution. We studied the direct introduction of a gaseous sample into a C18 reversed-phase column, followed by separation of the components by HPLC with UV detection. Since the chromatography was performed at high pressure, vapors readily dissolved in the eluent and the substances separated in the column as effectively as in liquid samples. Samples were injected into the column in two ways: a) through the valve without a flow stop; b) after stopping the flow and relieving all pressure. We showed that an injectable gas volume could reach 70% of column dead volume. When an injected gaseous sample volume was less than 10% of the column dead volume, the resulting peaks were symmetrical and the column efficiency was high.

Язык оригиналаанглийский
Страницы (с-по)167-170
Число страниц4
ЖурналJournal of Chromatography A
Том1501
DOI
СостояниеОпубликовано - 9 июн 2017

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