Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining

Maria V. Babak; Pauline Le Faouder; Xavier Trivelli; Gopalakrishnan Venkatesan; Stanislav I. Bezzubov; Mohammed Kajjout; Artem L. Gushchin; Muhammad Hanif; Olivier Poizat; Hervé Vezin; Christian Rolando

doi:10.1021/acs.inorgchem.9b03679

Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining

Maria V. Babak, Pauline Le Faouder, Xavier Trivelli, Gopalakrishnan Venkatesan, Stanislav I. Bezzubov, Mohammed Kajjout, Artem L. Gushchin, Muhammad Hanif, Olivier Poizat, Hervé Vezin, Christian Rolando

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

5 Цитирования (Scopus)

Аннотация

The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent Ru^II tris(bathophenanthroline disulfonate) complex (Ru(BPS)₃), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic Ru^II complexes Ru(BPS)₂(BP) and Ru(BPS)(BP)₂ containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)₃. It was shown by fluorescent and UV-vis measurements that novel Ru^II complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)₂ can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

Язык оригинала	английский
Страницы (с-по)	4527-4535
Число страниц	9
Журнал	Inorganic Chemistry
Том	59
Номер выпуска	7
DOI	https://doi.org/10.1021/acs.inorgchem.9b03679
Состояние	Опубликовано - 17 мар. 2020

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10.1021/acs.inorgchem.9b03679

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Babak, M. V., Le Faouder, P., Trivelli, X., Venkatesan, G., Bezzubov, S. I., Kajjout, M., Gushchin, A. L., Hanif, M., Poizat, O., Vezin, H., & Rolando, C. (2020). Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining. Inorganic Chemistry, 59(7), 4527-4535. https://doi.org/10.1021/acs.inorgchem.9b03679

@article{e85c03588e064be28f5fccba452b400e,

title = "Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining",

abstract = "The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).",

keywords = "POLYACRYLAMIDE GELS, SYPRO-RUBY, ELECTROCHEMILUMINESCENCE, ELECTROPHORESIS, PHOTOPHYSICS, ROUTE, WATER, ACIDS",

author = "Babak, {Maria V.} and {Le Faouder}, Pauline and Xavier Trivelli and Gopalakrishnan Venkatesan and Bezzubov, {Stanislav I.} and Mohammed Kajjout and Gushchin, {Artem L.} and Muhammad Hanif and Olivier Poizat and Herv{\'e} Vezin and Christian Rolando",

year = "2020",

month = mar,

day = "17",

doi = "10.1021/acs.inorgchem.9b03679",

language = "English",

volume = "59",

pages = "4527--4535",

journal = "Inorganic Chemistry",

issn = "0020-1669",

publisher = "American Chemical Society",

number = "7",

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Babak, MV, Le Faouder, P, Trivelli, X, Venkatesan, G, Bezzubov, SI, Kajjout, M, Gushchin, AL, Hanif, M, Poizat, O, Vezin, H & Rolando, C 2020, 'Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining', Inorganic Chemistry, том. 59, № 7, стр. 4527-4535. https://doi.org/10.1021/acs.inorgchem.9b03679

Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining. / Babak, Maria V.; Le Faouder, Pauline; Trivelli, Xavier и др.

В: Inorganic Chemistry, Том 59, № 7, 17.03.2020, стр. 4527-4535.

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

TY - JOUR

T1 - Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining

AU - Babak, Maria V.

AU - Le Faouder, Pauline

AU - Trivelli, Xavier

AU - Venkatesan, Gopalakrishnan

AU - Bezzubov, Stanislav I.

AU - Kajjout, Mohammed

AU - Gushchin, Artem L.

AU - Hanif, Muhammad

AU - Poizat, Olivier

AU - Vezin, Hervé

AU - Rolando, Christian

PY - 2020/3/17

Y1 - 2020/3/17

N2 - The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

AB - The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

KW - POLYACRYLAMIDE GELS

KW - SYPRO-RUBY

KW - ELECTROCHEMILUMINESCENCE

KW - ELECTROPHORESIS

KW - PHOTOPHYSICS

KW - ROUTE

KW - WATER

KW - ACIDS

UR - http://www.scopus.com/inward/record.url?scp=85082314450&partnerID=8YFLogxK

U2 - 10.1021/acs.inorgchem.9b03679

DO - 10.1021/acs.inorgchem.9b03679

M3 - Article

C2 - 32181663

AN - SCOPUS:85082314450

VL - 59

SP - 4527

EP - 4535

JO - Inorganic Chemistry

JF - Inorganic Chemistry

SN - 0020-1669

IS - 7

ER -