Genetically encoded fluorescent biosensors for biomedical applications

Vera S. Ovechkina, Suren M. Zakian, Sergey P. Medvedev, Kamila R. Valetdinova

Результат исследования: Научные публикации в периодических изданияхобзорная статьярецензирование

Аннотация

One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise ap-propriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summa-rizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discus-sion on the challenges, future trends, and potential inputs needed for improving them.

Язык оригиналаанглийский
Номер статьи1528
ЖурналBiomedicines
Том9
Номер выпуска11
DOI
СостояниеОпубликовано - ноя 2021

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