Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells

Ekaterina A. Potter, Evgenia V. Dolgova, Anastasia S. Proskurina, Yaroslav R. Efremov, Alexandra M. Minkevich, Aleksey S. Rozanov, Sergey E. Peltek, Valeriy P. Nikolin, Nelly A. Popova, Igor A. Seledtsov, Vladimir V. Molodtsov, Evgeniy L. Zavyalov, Oleg S. Taranov, Sergey I. Baiborodin, Alexander A. Ostanin, Elena R. Chernykh, Nikolay A. Kolchanov, Sergey S. Bogachev

Результат исследования: Научные публикации в периодических изданияхстатья

8 Цитирования (Scopus)

Аннотация

Using the ability of poorly differentiated cells to natively internalize fragments of extracellular double-stranded DNA as a marker, we isolated a tumorigenic subpopulation present in Krebs-2 ascites that demonstrated the features of tumor-inducing cancer stem cells. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, we identified a set of 168 genes specifically expressed in TAMRA-positive cells (tumor-initiating stem cells), these genes remaining silent in TAMRA-negative cancer cells. TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells. The observed expression differences between TAMRA+ and TAMRA- cells were validated by Real Time PCR. The results obtained corroborated the biological data that TAMRA+ murine Krebs-2 tumor cells are tumor-initiating stem cells. The approach developed can be applied to profile any poorly differentiated cell types that are capable of immanent internalization of double-stranded DNA.

Язык оригиналаанглийский
Страницы (с-по)9425-9441
Число страниц17
ЖурналOncotarget
Том8
Номер выпуска6
DOI
СостояниеОпубликовано - 7 фев 2017

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  • Цитировать

    Potter, E. A., Dolgova, E. V., Proskurina, A. S., Efremov, Y. R., Minkevich, A. M., Rozanov, A. S., Peltek, S. E., Nikolin, V. P., Popova, N. A., Seledtsov, I. A., Molodtsov, V. V., Zavyalov, E. L., Taranov, O. S., Baiborodin, S. I., Ostanin, A. A., Chernykh, E. R., Kolchanov, N. A., & Bogachev, S. S. (2017). Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells. Oncotarget, 8(6), 9425-9441. https://doi.org/10.18632/oncotarget.14116