TY - GEN
T1 - Functional study of potential regulatory SNPs (rs590352, rs11542583, rs3829202, rs78317230, rs2072580, rs4796672)
AU - Degtyareva, Arina
AU - Leberfarb, Elena
AU - Brusentsov, Ilya
AU - Kuzina, Tatiana
AU - Merkulova, Tatiana
PY - 2020/7
Y1 - 2020/7
N2 - In a previous study using a new bioinformatic approach based on Chip-Seq data with antibodies against various transcription factors and histone modifications, ChiA-PET, RNA-seq and ICGC data, 32 potentially regulatory single - nucleotide polymorphisms (rSNPs) were associated with colorectal cancer. In this study, a functional analysis of 6 of these polymorphisms was performed using EMSA and the luciferase reporter system. These nucleotide substitutions have been shown to alter the binding of nuclear extract proteins and influence the expression of the reporter gene.
AB - In a previous study using a new bioinformatic approach based on Chip-Seq data with antibodies against various transcription factors and histone modifications, ChiA-PET, RNA-seq and ICGC data, 32 potentially regulatory single - nucleotide polymorphisms (rSNPs) were associated with colorectal cancer. In this study, a functional analysis of 6 of these polymorphisms was performed using EMSA and the luciferase reporter system. These nucleotide substitutions have been shown to alter the binding of nuclear extract proteins and influence the expression of the reporter gene.
KW - Electrophoretic Mobility Shift Assay (EMSA)
KW - gene expression
KW - luciferase assay
KW - regulatory SNP (rSNP)
UR - http://www.scopus.com/inward/record.url?scp=85094823809&partnerID=8YFLogxK
UR - https://elibrary.ru/item.asp?id=44042377
U2 - 10.1109/CSGB51356.2020.9214780
DO - 10.1109/CSGB51356.2020.9214780
M3 - Conference contribution
AN - SCOPUS:85094823809
T3 - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
SP - 122
EP - 125
BT - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
Y2 - 6 July 2020 through 10 July 2020
ER -