Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev; Ekaterina V. Bondina; Sergey A. Dolgushin; Victoria S. Semeykina; Natalya N. Sankova; Ekaterina V. Parkhomchuk

doi:10.1117/12.2526387

Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, Ekaterina V. Parkhomchuk

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › статья в сборнике материалов конференции › научная › рецензирование

Аннотация

Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

Язык оригинала	английский
Название основной публикации	Clinical and Preclinical Optical Diagnostics II
Редакторы	J. Quincy Brown, Ton G. van Leeuwen
Издатель	SPIE
Число страниц	3
ISBN (электронное издание)	9781510628397
DOI	https://doi.org/10.1117/12.2526387
Состояние	Опубликовано - 1 янв 2019
Событие	Clinical and Preclinical Optical Diagnostics II 2019 - Munich, Германия Продолжительность: 23 июн 2019 → 25 июн 2019

Серия публикаций

Название	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Том	11073
ISSN (печатное издание)	1605-7422

Конференция

Конференция	Clinical and Preclinical Optical Diagnostics II 2019
Страна	Германия
Город	Munich
Период	23.06.2019 → 25.06.2019

Fingerprint

Цитировать

Shalaev, P. V., Bondina, E. V., Dolgushin, S. A., Semeykina, V. S., Sankova, N. N., & Parkhomchuk, E. V. (2019). Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. В J. Q. Brown, & T. G. van Leeuwen (Ред.), Clinical and Preclinical Optical Diagnostics II [110731W] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Том 11073). SPIE. https://doi.org/10.1117/12.2526387

Shalaev, Pavel V. ; Bondina, Ekaterina V. ; Dolgushin, Sergey A. ; Semeykina, Victoria S. ; Sankova, Natalya N. ; Parkhomchuk, Ekaterina V. / Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. Clinical and Preclinical Optical Diagnostics II. редактор / J. Quincy Brown ; Ton G. van Leeuwen. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{e3bdd7b69c664fa69b5bef334e2081d5,

title = "Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis",

abstract = "Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.",

keywords = "Cyanine dyes, Immunofluorescence analysis, Polystyrene microspheres, polystyrene microspheres, immunofluorescence analysis, cyanine dyes",

author = "Shalaev, {Pavel V.} and Bondina, {Ekaterina V.} and Dolgushin, {Sergey A.} and Semeykina, {Victoria S.} and Sankova, {Natalya N.} and Parkhomchuk, {Ekaterina V.}",

year = "2019",

month = jan,

day = "1",

doi = "10.1117/12.2526387",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Brown, {J. Quincy} and {van Leeuwen}, {Ton G.}",

booktitle = "Clinical and Preclinical Optical Diagnostics II",

address = "United States",

note = "Clinical and Preclinical Optical Diagnostics II 2019 ; Conference date: 23-06-2019 Through 25-06-2019",

}

Shalaev, PV, Bondina, EV, Dolgushin, SA, Semeykina, VS, Sankova, NN & Parkhomchuk, EV 2019, Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. в JQ Brown & TG van Leeuwen (ред.), Clinical and Preclinical Optical Diagnostics II., 110731W, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, том. 11073, SPIE, Clinical and Preclinical Optical Diagnostics II 2019, Munich, Германия, 23.06.2019. https://doi.org/10.1117/12.2526387

Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. / Shalaev, Pavel V.; Bondina, Ekaterina V.; Dolgushin, Sergey A.; Semeykina, Victoria S.; Sankova, Natalya N.; Parkhomchuk, Ekaterina V.

Clinical and Preclinical Optical Diagnostics II. ред. / J. Quincy Brown; Ton G. van Leeuwen. SPIE, 2019. 110731W (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Том 11073).

Результат исследования: Публикации в книгах, отчётах, сборниках, трудах конференций › статья в сборнике материалов конференции › научная › рецензирование

TY - GEN

T1 - Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

AU - Shalaev, Pavel V.

AU - Bondina, Ekaterina V.

AU - Dolgushin, Sergey A.

AU - Semeykina, Victoria S.

AU - Sankova, Natalya N.

AU - Parkhomchuk, Ekaterina V.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

AB - Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

KW - Cyanine dyes

KW - Immunofluorescence analysis

KW - Polystyrene microspheres

KW - polystyrene microspheres

KW - immunofluorescence analysis

KW - cyanine dyes

UR - http://www.scopus.com/inward/record.url?scp=85073749856&partnerID=8YFLogxK

U2 - 10.1117/12.2526387

DO - 10.1117/12.2526387

M3 - Conference contribution

AN - SCOPUS:85073749856

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Clinical and Preclinical Optical Diagnostics II

A2 - Brown, J. Quincy

A2 - van Leeuwen, Ton G.

PB - SPIE

T2 - Clinical and Preclinical Optical Diagnostics II 2019

Y2 - 23 June 2019 through 25 June 2019

ER -

Shalaev PV, Bondina EV, Dolgushin SA, Semeykina VS, Sankova NN , Parkhomchuk EV. Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. В Brown JQ, van Leeuwen TG, редакторы, Clinical and Preclinical Optical Diagnostics II. SPIE. 2019. 110731W. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2526387

Доступ к документу

10.1117/12.2526387

Link to publication in Scopus