First report on B chromosome content in a reptilian species: the case of Anolis carolinensis

Ilya G. Kichigin, Artem P. Lisachov, Massimo Giovannotti, Alex I. Makunin, Marsel R. Kabilov, Patricia C.M. O’Brien, Malcolm A. Ferguson-Smith, Alexander S. Graphodatsky, Vladimir A. Trifonov

Результат исследования: Научные публикации в периодических изданияхстатья

2 Цитирования (Scopus)

Аннотация

Supernumerary elements of the genome are often called B chromosomes. They usually consist of various autosomal sequences and, because of low selective pressure, are mostly pseudogenized and contain many repeats. There are numerous reports on B chromosomes in mammals, fish, invertebrates, plants, and fungi, but only a few of them have been studied using sequencing techniques. However, reptilian supernumerary chromosomes have been detected only cytogenetically and never sequenced or analyzed at the molecular level. One model squamate species with available genome sequence is Anolis carolinensis. The scope of the present article is to describe the genetic content of A. carolinensis supernumerary chromosomes. In this article, we confirm the presence of B chromosomes in this species by reverse painting and synaptonemal complex analysis. We applied low-pass high-throughput sequencing to analyze flow-sorted B chromosomes. Anole B chromosomes exhibit similar traits to other supernumerary chromosomes from different taxons: they contain two genes related to cell division control (INCENP and SPIRE2), are enriched in specific repeats, and show a high degree of pseudogenization. Therefore, the present study confirms that reptilian B chromosomes resemble supernumerary chromosomes of other taxons.

Язык оригиналаанглийский
Страницы (с-по)13-21
Число страниц9
ЖурналMolecular Genetics and Genomics
Том294
Номер выпуска1
DOI
СостояниеОпубликовано - 11 фев 2019

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Kichigin, I. G., Lisachov, A. P., Giovannotti, M., Makunin, A. I., Kabilov, M. R., O’Brien, P. C. M., ... Trifonov, V. A. (2019). First report on B chromosome content in a reptilian species: the case of Anolis carolinensis. Molecular Genetics and Genomics, 294(1), 13-21. https://doi.org/10.1007/s00438-018-1483-9