Experimental and morphological justification lymphotropic phytotechnology of functional food when aging

Владимир Николаевич Горчаков, Yurii Kolmogorov, Alexandr Fartukov

Результат исследования: Материалы конференцийтезисырецензирование


Background: The modern medicine estimated a food role in human life, attracting knowledge of anatomy, biochemistry and pharmacology. Accumulation of knowledge of lymphatic system allowed to integrate a lymphology with a nutritiology and pharmacology. It promoted emergence of the new direction a lymphonutritiology. This direction studies biologically active compounds, having lymphotropic properties. A phytocomponent is an integral part of biologically active food additives and foodstuff. Phytonutrients have high therapeutic value. We use bioactive compounds (bioflavonoids) for increase in protective function of peripheral lymphoid organs and management through lymphatic system for improvement of an organism, especially, at age-associated pathology [1]. There is basic a justification and implementation of lymphotropic technologies which could correct structure of lymph nodes and offer the real prospect of fight against aging of lymphatic (lymphoid) system.
The purpose of study is experimentally to prove positive influence of phytosorption means on structure and function of senile lymph nodes.
Methods: We developed the phytosorption complex including herbs of Siberia and a compound of food fibres. Biologically active compounds (bioflavonoids, microelements and other nutrients) are means of hypotoxicity that it is important for functional food [2]. The receipt of phytocomposition is the subject "know-how". White rats of Wistar with the natural rate of aging participated in an experiment. The age of rats was 1.5–2 years (old). We expected a dose of phytocomposition. The daily dose averaged 0.1–0.2 g/kg. The phytosupplements was added to the main forage of animals within 1 month.
Mesenteric, inguinal and tracheobronchial lymph nodes investigated by a morphological method. Lymph nodes fixed in 10% neutral formalin. After fixing we adhered to the classical scheme of washing, dehydration, imbibition with a xylol, paraffin. Histologic sections of lymph nodes painted hematoxylin and eosine, trichromatic paint by C. Masson. We defined the content of trace elements (Se, Mn, Fe, Cu, Zn) in vegetable substance and lymph nodes by the X-ray fluorescence analysis with use of synchrotron radiation. In work used the equipment of Institute of nuclear physics of the SB RAS (Novosibirsk) supported by project number RFMEFI62119X0022 [3]. Statistical data processing was performed with licensed software package StatPlus Pro 2009, AnalystSoft Inc.
Results: Lymphatic system reduces the structural and functional reserves when aging [1]. There is an age reduction of the lymphatic bed and compartments of lymph nodes because of involution of lymphoid tissue and substitution by connective or fatty tissue [4]. Aging is characterized by trace elements deficiency with decrease of the activity of enzymes, cellular proliferation and loss of lymphoid-microelement association [5].
The result of age transformation leads to decrease in drainage and immune function of lymph nodes and increase in risk of developing diseases. So it is the reason of search of the means increasing function of peripheral lymphoid organs. The obtained data show presence of lymphotropic properties of the applied phytomeans as element of functional food. Lymphotropic properties of phytomeans come down to the following positive effects at the level of lymphatic system (lymph nodes) during the senile period.
We observed effect a lymphostimulation. Lymph passage speed through a lymph node increases by 1.3–1.5 times at the majority of old animals after phytotherapy. Phytomeans is causing the structural modifying effect on lymph nodes structure. This effect is expressed in selective increase or reduction of the sizes of structural and functional zones at old animals. Intake of phytocomposition provides the lymphoprotection principle. Bioflavonoids of medicinal plants strengthen function of excretory organs. It reduces toxic pressure on lymphatic system. For the first time we noted lymphoproliferation effect as a result of phytotherapy. There is a formation of temporary compensatory lymphoid structures – lymphoid follicles outside and into a lymph node. New lymphoid follicles are necessary for increase in immune function of peripheral lymphoid organs. Herbs are a source of additional trace elements. Intake of medicinal plants fill micronutrient deficiency and positively affects structure and function of lymph nodes when aging.
The received results became a basis for development of phytolymphotropic technologies of prevention and rehabilitation in sanatoria and health resorts. Herbs in the form of phytosorption complexes, dietary supplements to food proved the efficiency as elements of functional food in "background" therapy age-associated diseases. The positive effect is connected with increase in nonspecific resistance of an organism.
Conclusion: The age-caused deterioration in function of peripheral lymphoid tissue puts before need to correct structure and to increase function of lymph nodes. It can be reached use of phytolymphotropic technologies of functional food. It is shown that biologically active compounds of herbs lead to strengthening of drainage and immune functions of lymph nodes when aging. The main active ingredients of plants are bioflavonoids and microelements. They have positive therapeutic effects on organs and systems including on lymphatic system. Phytomeans are capable to correct structure of lymph nodes and offer the real prospect of fight against various diseases and lymphatic (lymphoid) aging.
1. Borodin Yu.I., Lymphatic system and ageing. Fundamental researches 2011; 5: 11–15. URL: http://fundamental-research.ru/ru/article/view?id=21252
2. Martirosyan D.M., Singh J., A new definition of functional food by FFC: what makes a new definition unique? Functional Foods in Health and Disease 2015; 5(6): 209–223
3. Piminov P.A., Synchrotron Radiation Research and Application at VEPP-4. Physics Procedia 2016; 84: 19–26. DOI:10.1016/j.phpro.2016.11.005
4. Gorchakova O., Kolmogorov Yu., Gorchakov V., Demchenko G., Interrelation of trace elements and the structural organization of lymph nodes at young and senile age. Archiv Euromedica 2020; 10(2): P.22–25. http://dx.doi.org/10.35630/2199-885X/2020/10/2.6
Язык оригиналаанглийский
Число страниц2
СостояниеОпубликовано - 2021
СобытиеFFC’s 29th International Conference on Functional Foods “Can Functional Foods and Bioactive Compounds Reduce the Risk of Chronic and Viral Diseases?”, - Dallas, Соединенные Штаты Америки
Продолжительность: 29 апр. 202130 апр. 2021


КонференцияFFC’s 29th International Conference on Functional Foods “Can Functional Foods and Bioactive Compounds Reduce the Risk of Chronic and Viral Diseases?”,
Сокращенный заголовокFFC’s 29th International Conference
Страна/TерриторияСоединенные Штаты Америки

Предметные области OECD FOS+WOS