Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

A. Ya Bolsunovsky; D. V. Dementyev; T. S. Frolova; E. A. Trofimova; E. M. Iniatkina; S. A. Vasilyev; O. I. Sinitsyna

doi:10.1134/S1607672919060024

Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

A. Ya Bolsunovsky, D. V. Dementyev, T. S. Frolova, E. A. Trofimova, E. M. Iniatkina, S. A. Vasilyev, O. I. Sinitsyna

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

Аннотация

Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

Язык оригинала	английский
Страницы (с-по)	362-366
Число страниц	5
Журнал	Doklady. Biochemistry and biophysics
Том	489
Номер выпуска	1
DOI	https://doi.org/10.1134/S1607672919060024
Состояние	Опубликовано - 1 нояб. 2019

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10.1134/S1607672919060024

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@article{a1cf6fb749024b52ae40db60de2da4b4,

title = "Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings",

abstract = "Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.",

keywords = "DNA Damage, Dose-Response Relationship, Radiation, Gamma Rays/adverse effects, Onions/genetics, Seedlings/genetics, CELLS, REPAIR, COMET ASSAY",

author = "Bolsunovsky, {A. Ya} and Dementyev, {D. V.} and Frolova, {T. S.} and Trofimova, {E. A.} and Iniatkina, {E. M.} and Vasilyev, {S. A.} and Sinitsyna, {O. I.}",

year = "2019",

month = nov,

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doi = "10.1134/S1607672919060024",

language = "English",

volume = "489",

pages = "362--366",

journal = "Doklady Biochemistry and Biophysics",

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Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings. / Bolsunovsky, A. Ya; Dementyev, D. V.; Frolova, T. S. и др.

В: Doklady. Biochemistry and biophysics, Том 489, № 1, 01.11.2019, стр. 362-366.

Результат исследования: Научные публикации в периодических изданиях › статья › рецензирование

TY - JOUR

T1 - Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

AU - Bolsunovsky, A. Ya

AU - Dementyev, D. V.

AU - Frolova, T. S.

AU - Trofimova, E. A.

AU - Iniatkina, E. M.

AU - Vasilyev, S. A.

AU - Sinitsyna, O. I.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

AB - Abstract: The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

KW - DNA Damage

KW - Dose-Response Relationship, Radiation

KW - Gamma Rays/adverse effects

KW - Onions/genetics

KW - Seedlings/genetics

KW - CELLS

KW - REPAIR

KW - COMET ASSAY

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U2 - 10.1134/S1607672919060024

DO - 10.1134/S1607672919060024

M3 - Article

C2 - 32130600

AN - SCOPUS:85081042218

VL - 489

SP - 362

EP - 366

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - 1

ER -

Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

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