TY - GEN
T1 - Dual-agonist Optical Stimulation of Platelets Results in Increased and Reliable Activation
AU - Spiryova, Darya
AU - Vorob'ev, Alexei
AU - Moskalensky, Alexander E.
PY - 2020/7
Y1 - 2020/7
N2 - Activation of blood platelets is the main process in normal hemostasis. Unfortunately, it also makes a great contribution to the development of cardiovascular diseases. In this work, we study activation dynamics using cytosolic calcium probe. To increase the accuracy of dynamic measurements, we present a method for optical activation of single platelets in suspension. It is achieved by the use of photolabile "caged"activation agonists. In our earlier works, the use of such compounds resulted in precise measurement of early activation dynamics. However, relatively large irradiation dose was needed to achieve the activation of significant fraction of cells. Here we show that dual-agonist stimulation dramatically increases the activation probability, resulting in increased and reliable activation with 10-to-50-times less irradiation dose as compared to our previous report.
AB - Activation of blood platelets is the main process in normal hemostasis. Unfortunately, it also makes a great contribution to the development of cardiovascular diseases. In this work, we study activation dynamics using cytosolic calcium probe. To increase the accuracy of dynamic measurements, we present a method for optical activation of single platelets in suspension. It is achieved by the use of photolabile "caged"activation agonists. In our earlier works, the use of such compounds resulted in precise measurement of early activation dynamics. However, relatively large irradiation dose was needed to achieve the activation of significant fraction of cells. Here we show that dual-agonist stimulation dramatically increases the activation probability, resulting in increased and reliable activation with 10-to-50-times less irradiation dose as compared to our previous report.
KW - caged ADP
KW - caged epinephrine
KW - cytosolic calcium
KW - Platelet activation
UR - http://www.scopus.com/inward/record.url?scp=85094853155&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=45181727
U2 - 10.1109/CSGB51356.2020.9214621
DO - 10.1109/CSGB51356.2020.9214621
M3 - Conference contribution
AN - SCOPUS:85094853155
T3 - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
SP - 106
EP - 109
BT - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020
Y2 - 6 July 2020 through 10 July 2020
ER -