DNA is a New Target of Parp3

E. A. Belousova, A. A. Ishchenko, O. I. Lavrik

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

34 Цитирования (Scopus)

Аннотация

Most members of the poly(ADP-ribose)polymerase family, PARP family, have a catalytic activity that involves the transfer of ADP-ribose from a beta-NAD+-molecule to protein acceptors. It was recently discovered by Talhaoui et al. that DNA-dependent PARP1 and PARP2 can also modify DNA. Here, we demonstrate that DNA-dependent PARP3 can modify DNA and form a specific primed structure for further use by the repair proteins. We demonstrated that gapped DNA that was ADP-ribosylated by PARP3 could be ligated to double-stranded DNA by DNA ligases. Moreover, this ADP-ribosylated DNA could serve as a primed DNA substrate for PAR chain elongation by the purified proteins PARP1 and PARP2 as well as by cell-free extracts. We suggest that this ADP-ribose modification can be involved in cellular pathways that are important for cell survival in the process of double-strand break formation.

Язык оригиналаанглийский
Номер статьи4176
Страницы (с-по)4176
Число страниц12
ЖурналScientific Reports
Том8
Номер выпуска1
DOI
СостояниеОпубликовано - 8 мар 2018

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