Deterministic versus stochastic model of reprogramming: New evidence from cellular barcoding technique

Anastasia M. Yunusova, Veniamin S. Fishman, Gennady V. Vasiliev, Nariman R. Battulin

Результат исследования: Научные публикации в периодических изданияхстатья

4 Цитирования (Scopus)

Аннотация

Factor-mediated reprogramming of somatic cells towards pluripotency is a low-efficiency process during which only small subsets of cells are successfully reprogrammed. Previous analyses of the determinants of the reprogramming potential are based on average measurements across a large population of cells or on monitoring a relatively small number of single cells with live imaging. Here, we applied lentiviral genetic barcoding, a powerful tool enabling the identification of familiar relationships in thousands of cells. High-throughput sequencing of barcodes from successfully reprogrammed cells revealed a significant number of barcodes from related cells. We developed a computer model, according to which a probability of synchronous reprogramming of sister cells equals 10-30%. We conclude that the reprogramming success is pre-established in some particular cells and, being a heritable trait, can be maintained through cell division. Thus, reprogramming progresses in a deterministic manner, at least at the level of cell lineages.

Язык оригиналаанглийский
Номер статьи160311
Число страниц13
ЖурналOpen Biology
Том7
Номер выпуска4
DOI
СостояниеОпубликовано - апр 2017

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