TY - JOUR
T1 - Degradation of core-shell Au@SiO2 nanoparticles in biological media
AU - Ryabchikova, Elena I.
AU - Chelobanov, Boris P.
AU - Parkhomenko, Roman G.
AU - Korchagina, Kseniya V.
AU - Basova, Tamara V.
PY - 2017/8
Y1 - 2017/8
N2 - In this work, the effect of a cell culture medium on the integrity of mesoporous silica shell of gold-core nanoparticles (Au@SiO2 NPs) and their behavior in cells of HeLa, MDA-MB and BT cultures were studied using the method of transmission electron microscopy. It was shown that degradation of Au@SiO2 NPs takes place in the culture medium. The presence of the cells and serum does not influence on the process of the mesoporous silica layer degradation. The presence of both damaged and undamaged NPs in endosomes and lysosomes is related to different time of NPs’ location outside of the cell, i.e. in culture medium. The analysis of the cell ultrastructure leads to a conclusion that the internal conditions in endosomes/lysosomes do not influence on the integrity of Au@SiO2 NPs.
AB - In this work, the effect of a cell culture medium on the integrity of mesoporous silica shell of gold-core nanoparticles (Au@SiO2 NPs) and their behavior in cells of HeLa, MDA-MB and BT cultures were studied using the method of transmission electron microscopy. It was shown that degradation of Au@SiO2 NPs takes place in the culture medium. The presence of the cells and serum does not influence on the process of the mesoporous silica layer degradation. The presence of both damaged and undamaged NPs in endosomes and lysosomes is related to different time of NPs’ location outside of the cell, i.e. in culture medium. The analysis of the cell ultrastructure leads to a conclusion that the internal conditions in endosomes/lysosomes do not influence on the integrity of Au@SiO2 NPs.
KW - Eukaryotic cells
KW - Gold core–mesoporous silica shell nanoparticles
KW - Mesoporous silica
KW - Transmission electron microscopy
KW - GOLD NANOPARTICLES
KW - DRUG-DELIVERY
KW - BEHAVIOR
KW - SURFACE CHARACTERISTICS
KW - Gold core-mesoporous silica shell nanoparticles
KW - MESOPOROUS SILICA NANOPARTICLES
KW - NANORODS
KW - CANCER-THERAPY
KW - GROWTH
KW - PLASMON RESONANCE
KW - GEOMETRY
UR - http://www.scopus.com/inward/record.url?scp=85017422890&partnerID=8YFLogxK
U2 - 10.1016/j.micromeso.2017.04.006
DO - 10.1016/j.micromeso.2017.04.006
M3 - Article
AN - SCOPUS:85017422890
VL - 248
SP - 46
EP - 53
JO - Microporous and Mesoporous Materials
JF - Microporous and Mesoporous Materials
SN - 1387-1811
ER -