Concatenation of transgenic DNA: Random or orchestrated?

Alexander Smirnov, Nariman Battulin

Результат исследования: Научные публикации в периодических изданияхобзорная статьярецензирование

Аннотация

Generation of transgenic organisms by pronuclear microinjection has become a routine procedure. However, while the process of DNA integration in the genome is well understood, we still do not know much about the recombination between transgene molecules that happens in the first moments after DNA injection. Most of the time, injected molecules are joined together in head-to-tail tandem repeats—the so-called concatemers. In this review, we focused on the possible concatenation mechanisms and how they could be studied with genetic reporters tracking individual copies in concatemers. We also discuss various features of concatemers, including palindromic junctions and repeat-induced gene silencing (RIGS). Finally, we speculate how cooperation of DNA repair pathways creates a multicopy concatenated insert.

Язык оригиналаанглийский
Номер статьи1969
ЖурналGenes
Том12
Номер выпуска12
DOI
СостояниеОпубликовано - дек 2021

Предметные области OECD FOS+WOS

  • 1.06 БИОЛОГИЧЕСКИЕ НАУКИ
  • 1.06.KM ГЕНЕТИКА И НАСЛЕДСТВЕННОСТЬ

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