Comparative Analysis of Nucleotide Fluorescent Analogs for Registration of DNA Conformational Changes Induced by Interaction with Formamidopyrimidine-DNA Glycosylase Fpg

A. A. Kuznetsova, O. A. Kladova, Nicolas P.F. Barthes, Benoit Y. Michel, Alain Burger, O. S. Fedorova, N. A. Kuznetsov

Результат исследования: Научные публикации в периодических изданияхстатья

Аннотация

DNA-substrates containing fluorescent DNA base analogs are widely used to study protein–nucleic acid interactions. In the case of DNA-recognizing enzymes, this approach allows one to register conformational changes in DNA during the formation of enzyme–substrate complexes. An important part of such research is the design of model DNA substrates, which includes both the photophysical properties of the fluorescent groups and their location relative to a specific recognition site, namely, in the same chain on the 5′-, 3′-side or in the complementary chain opposite the specific site. In this work, we report a comparative study of the sensitivity of various fluorescent DNA base analogs, such as 2-aminopurine (aPu), pyrrolocytosine (CPy), 1,3-diaza-2-oxophenoxazine (tCO) and 3-hydroxychromone (3HC), to conformational transformations of DNA in the process of interaction with formamidopyrimidine-DNA glycosylase (Fpg) from Escherichia coli.

Язык оригиналаанглийский
Страницы (с-по)591-598
Число страниц8
ЖурналRussian Journal of Bioorganic Chemistry
Том45
Номер выпуска6
DOI
СостояниеОпубликовано - 1 ноя 2019

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