Communication: Alamethicin can capture lipid-like molecules in the membrane

Ekaterina F. Afanasyeva, Victoria N. Syryamina, Sergei A. Dzuba

Результат исследования: Научные публикации в периодических изданияхстатья

5 Цитирования (Scopus)

Аннотация

Alamethicin (Alm) is a 19-mer antimicrobial peptide produced by fungus Trichoderma viride. Above a threshold concentration, Alm forms pores across the membrane, providing a mechanism of its antimicrobial action. Here we show that at a small concentration which is below the threshold value, Alm participates in formation of nanoscale lipid-mediated clusters of guest lipid-like molecules in the membrane. These results are obtained by electron spin echo (ESE) technique - a pulsed version of electron paramagnetic resonance - on spin-labeled stearic acid in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayer with Alm added at 1/200 peptide-to-lipid ratio. ESE decay measurements are interpreted assuming that stearic acid molecules in the membrane are assembling around the Alm molecule. One may suggest that this Alm capturing effect on the guest lipid-like molecules could be important for the peptide antimicrobial action.

Язык оригиналаанглийский
Номер статьи011103
Страницы (с-по)011103
Число страниц4
ЖурналJournal of Chemical Physics
Том146
Номер выпуска1
DOI
СостояниеОпубликовано - 7 янв 2017

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