Co-expression profile of TNF membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets

Alina Alshevskaya, Julia Lopatnikova, Julia Zhukova, Oksana Chumasova, Nadezhda Shkaruba, Aleksey Sizikov, Irina Evsegneeva, Victor Gladkikh, Aleksander Karaulov, Sergey V. Sennikov

Результат исследования: Научные публикации в периодических изданияхстатья

Выдержка

Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4%), moderate (n = 30, 46.9%), and low (n = 12, 18.8%) disease activity. Coexpression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2-cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among naïve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity.

Язык оригиналаанглийский
Номер статьи288
ЖурналInternational Journal of Molecular Sciences
Том21
Номер выпуска1
DOI
СостояниеОпубликовано - 1 янв 2020

Отпечаток

Receptors, Tumor Necrosis Factor, Type I
arthritis
necrosis
T-cells
set theory
Rheumatoid Arthritis
tumors
Tumor Necrosis Factor-alpha
Receptors, Tumor Necrosis Factor, Type II
membranes
Membranes
profiles
cells
Health
Data storage equipment
Helper-Inducer T-Lymphocytes
T-Lymphocytes
Flow cytometry
health
Tissue Donors

Цитировать

Alshevskaya, Alina ; Lopatnikova, Julia ; Zhukova, Julia ; Chumasova, Oksana ; Shkaruba, Nadezhda ; Sizikov, Aleksey ; Evsegneeva, Irina ; Gladkikh, Victor ; Karaulov, Aleksander ; Sennikov, Sergey V. / Co-expression profile of TNF membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets. В: International Journal of Molecular Sciences. 2020 ; Том 21, № 1.
@article{c6bd02617c9c40a19fb1adbd5317b2db,
title = "Co-expression profile of TNF membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets",
abstract = "Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4{\%}), moderate (n = 30, 46.9{\%}), and low (n = 12, 18.8{\%}) disease activity. Coexpression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2-cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among na{\"i}ve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity.",
keywords = "Co-expression, Cytokine receptors, Disease activity, Rheumatoid arthritis, TNF",
author = "Alina Alshevskaya and Julia Lopatnikova and Julia Zhukova and Oksana Chumasova and Nadezhda Shkaruba and Aleksey Sizikov and Irina Evsegneeva and Victor Gladkikh and Aleksander Karaulov and Sennikov, {Sergey V.}",
year = "2020",
month = "1",
day = "1",
doi = "10.3390/ijms21010288",
language = "English",
volume = "21",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "1",

}

Alshevskaya, A, Lopatnikova, J, Zhukova, J, Chumasova, O, Shkaruba, N, Sizikov, A, Evsegneeva, I, Gladkikh, V, Karaulov, A & Sennikov, SV 2020, 'Co-expression profile of TNF membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets', International Journal of Molecular Sciences, том. 21, № 1, 288. https://doi.org/10.3390/ijms21010288

Co-expression profile of TNF membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets. / Alshevskaya, Alina; Lopatnikova, Julia; Zhukova, Julia; Chumasova, Oksana; Shkaruba, Nadezhda; Sizikov, Aleksey; Evsegneeva, Irina; Gladkikh, Victor; Karaulov, Aleksander; Sennikov, Sergey V.

В: International Journal of Molecular Sciences, Том 21, № 1, 288, 01.01.2020.

Результат исследования: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Co-expression profile of TNF membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets

AU - Alshevskaya, Alina

AU - Lopatnikova, Julia

AU - Zhukova, Julia

AU - Chumasova, Oksana

AU - Shkaruba, Nadezhda

AU - Sizikov, Aleksey

AU - Evsegneeva, Irina

AU - Gladkikh, Victor

AU - Karaulov, Aleksander

AU - Sennikov, Sergey V.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4%), moderate (n = 30, 46.9%), and low (n = 12, 18.8%) disease activity. Coexpression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2-cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among naïve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity.

AB - Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4%), moderate (n = 30, 46.9%), and low (n = 12, 18.8%) disease activity. Coexpression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2-cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among naïve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity.

KW - Co-expression

KW - Cytokine receptors

KW - Disease activity

KW - Rheumatoid arthritis

KW - TNF

UR - http://www.scopus.com/inward/record.url?scp=85077599238&partnerID=8YFLogxK

U2 - 10.3390/ijms21010288

DO - 10.3390/ijms21010288

M3 - Article

C2 - 31906244

AN - SCOPUS:85077599238

VL - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 1

M1 - 288

ER -