Chondroitin sulfate content and decorin expression in glioblastoma are associated with proliferative activity of glioma cells and disease prognosis

Alexandra Y. Tsidulko, Galina M. Kazanskaya, Alexander M. Volkov, Anastasia V. Suhovskih, Roman S. Kiselev, Vyacheslav V. Kobozev, Alexei S. Gaytan, Alexei L. Krivoshapkin, Svetlana V. Aidagulova, Elvira V. Grigorieva

Результат исследования: Научные публикации в периодических изданияхстатья

Выдержка

Chondroitin sulfate proteoglycans (CSPGs) are important components of brain extracellular matrix (ECM), although their contribution in gliomagenesis remains underinvestigated. Here, both chondroitin sulfate (CS) content/distribution and expression of a number of CSPG core proteins were studied in glioblastoma multiforme (GBM) tumours with different prognosis (n = 40) using immunohistochemistry and RT-PCR analysis. Survival rates for clinically different patient groups were compared using the Kaplan-Meier analysis and univariate Cox model. CS content was increased in 60–65% of studied GBM tumours and distributed heterogeneously, mainly at perinecrotic and perivascular zones rather than tumour cells with specific morphology. CS accumulation, especially in the tumour extracellular matrix, was positively associated with the proliferative activity of GBM cells according to theKi67 index (p < 0.01) but revealed no significant association with age or sex of the patients, tumour localisation, relapse or disease outcome. The increase in CS content in GBM tumours was accompanied by upregulation of decorin (1.5-fold), biglycan (3-fold) and serglycin (2-fold) expression (p < 0.05), while only decorin expression level was negatively associated with the overall survival rate of the GBM patients (p < 0.05). These results demonstrate a contribution of CS to high intratumoural heterogeneity of GBM and suggest CS content and decorin expression for further investigation as potential microenvironmental glycomarkers/targets for GBM diagnostics and treatment.

Язык оригиналаанглийский
Страницы (с-по)147-155
Число страниц9
ЖурналCell and Tissue Research
Том379
Номер выпуска1
Ранняя дата в режиме онлайн26 ноя 2019
DOI
СостояниеОпубликовано - 1 янв 2020

Отпечаток

Decorin
Chondroitin Sulfates
Glioblastoma
Glioma
Chondroitin Sulfate Proteoglycans
Neoplasms
Extracellular Matrix
Survival Rate
Biglycan
Kaplan-Meier Estimate
Proportional Hazards Models
Up-Regulation
Immunohistochemistry
Recurrence
Polymerase Chain Reaction
Brain

Цитировать

Tsidulko, Alexandra Y. ; Kazanskaya, Galina M. ; Volkov, Alexander M. ; Suhovskih, Anastasia V. ; Kiselev, Roman S. ; Kobozev, Vyacheslav V. ; Gaytan, Alexei S. ; Krivoshapkin, Alexei L. ; Aidagulova, Svetlana V. ; Grigorieva, Elvira V. / Chondroitin sulfate content and decorin expression in glioblastoma are associated with proliferative activity of glioma cells and disease prognosis. В: Cell and Tissue Research. 2020 ; Том 379, № 1. стр. 147-155.
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title = "Chondroitin sulfate content and decorin expression in glioblastoma are associated with proliferative activity of glioma cells and disease prognosis",
abstract = "Chondroitin sulfate proteoglycans (CSPGs) are important components of brain extracellular matrix (ECM), although their contribution in gliomagenesis remains underinvestigated. Here, both chondroitin sulfate (CS) content/distribution and expression of a number of CSPG core proteins were studied in glioblastoma multiforme (GBM) tumours with different prognosis (n = 40) using immunohistochemistry and RT-PCR analysis. Survival rates for clinically different patient groups were compared using the Kaplan-Meier analysis and univariate Cox model. CS content was increased in 60–65{\%} of studied GBM tumours and distributed heterogeneously, mainly at perinecrotic and perivascular zones rather than tumour cells with specific morphology. CS accumulation, especially in the tumour extracellular matrix, was positively associated with the proliferative activity of GBM cells according to theKi67 index (p < 0.01) but revealed no significant association with age or sex of the patients, tumour localisation, relapse or disease outcome. The increase in CS content in GBM tumours was accompanied by upregulation of decorin (1.5-fold), biglycan (3-fold) and serglycin (2-fold) expression (p < 0.05), while only decorin expression level was negatively associated with the overall survival rate of the GBM patients (p < 0.05). These results demonstrate a contribution of CS to high intratumoural heterogeneity of GBM and suggest CS content and decorin expression for further investigation as potential microenvironmental glycomarkers/targets for GBM diagnostics and treatment.",
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Chondroitin sulfate content and decorin expression in glioblastoma are associated with proliferative activity of glioma cells and disease prognosis. / Tsidulko, Alexandra Y.; Kazanskaya, Galina M.; Volkov, Alexander M.; Suhovskih, Anastasia V.; Kiselev, Roman S.; Kobozev, Vyacheslav V.; Gaytan, Alexei S.; Krivoshapkin, Alexei L.; Aidagulova, Svetlana V.; Grigorieva, Elvira V.

В: Cell and Tissue Research, Том 379, № 1, 01.01.2020, стр. 147-155.

Результат исследования: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Chondroitin sulfate content and decorin expression in glioblastoma are associated with proliferative activity of glioma cells and disease prognosis

AU - Tsidulko, Alexandra Y.

AU - Kazanskaya, Galina M.

AU - Volkov, Alexander M.

AU - Suhovskih, Anastasia V.

AU - Kiselev, Roman S.

AU - Kobozev, Vyacheslav V.

AU - Gaytan, Alexei S.

AU - Krivoshapkin, Alexei L.

AU - Aidagulova, Svetlana V.

AU - Grigorieva, Elvira V.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Chondroitin sulfate proteoglycans (CSPGs) are important components of brain extracellular matrix (ECM), although their contribution in gliomagenesis remains underinvestigated. Here, both chondroitin sulfate (CS) content/distribution and expression of a number of CSPG core proteins were studied in glioblastoma multiforme (GBM) tumours with different prognosis (n = 40) using immunohistochemistry and RT-PCR analysis. Survival rates for clinically different patient groups were compared using the Kaplan-Meier analysis and univariate Cox model. CS content was increased in 60–65% of studied GBM tumours and distributed heterogeneously, mainly at perinecrotic and perivascular zones rather than tumour cells with specific morphology. CS accumulation, especially in the tumour extracellular matrix, was positively associated with the proliferative activity of GBM cells according to theKi67 index (p < 0.01) but revealed no significant association with age or sex of the patients, tumour localisation, relapse or disease outcome. The increase in CS content in GBM tumours was accompanied by upregulation of decorin (1.5-fold), biglycan (3-fold) and serglycin (2-fold) expression (p < 0.05), while only decorin expression level was negatively associated with the overall survival rate of the GBM patients (p < 0.05). These results demonstrate a contribution of CS to high intratumoural heterogeneity of GBM and suggest CS content and decorin expression for further investigation as potential microenvironmental glycomarkers/targets for GBM diagnostics and treatment.

AB - Chondroitin sulfate proteoglycans (CSPGs) are important components of brain extracellular matrix (ECM), although their contribution in gliomagenesis remains underinvestigated. Here, both chondroitin sulfate (CS) content/distribution and expression of a number of CSPG core proteins were studied in glioblastoma multiforme (GBM) tumours with different prognosis (n = 40) using immunohistochemistry and RT-PCR analysis. Survival rates for clinically different patient groups were compared using the Kaplan-Meier analysis and univariate Cox model. CS content was increased in 60–65% of studied GBM tumours and distributed heterogeneously, mainly at perinecrotic and perivascular zones rather than tumour cells with specific morphology. CS accumulation, especially in the tumour extracellular matrix, was positively associated with the proliferative activity of GBM cells according to theKi67 index (p < 0.01) but revealed no significant association with age or sex of the patients, tumour localisation, relapse or disease outcome. The increase in CS content in GBM tumours was accompanied by upregulation of decorin (1.5-fold), biglycan (3-fold) and serglycin (2-fold) expression (p < 0.05), while only decorin expression level was negatively associated with the overall survival rate of the GBM patients (p < 0.05). These results demonstrate a contribution of CS to high intratumoural heterogeneity of GBM and suggest CS content and decorin expression for further investigation as potential microenvironmental glycomarkers/targets for GBM diagnostics and treatment.

KW - Chondroitin sulfate proteoglycan

KW - Decorin

KW - Extracellular matrix

KW - Glioblastoma multiforme

KW - Tumour microenvironment

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U2 - 10.1007/s00441-019-03127-2

DO - 10.1007/s00441-019-03127-2

M3 - Article

C2 - 31773303

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VL - 379

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JO - Cell and Tissue Research

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