@article{77a98c7f88f54a909f2916a62e2b966b,
title = "Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus",
abstract = "Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.",
keywords = "Cell receptors, Cellular immune response, CRISPR/Cas9, CRISPR/Cas9 genome screening, Influenza virus, RNA processing factors, Cell Line, Humans, Gene Editing/methods, Virus Internalization, CRISPR-Cas Systems/genetics, Influenza A virus/genetics, Animals, Virus Replication, Chickens, Immunity, Cellular",
author = "Daria Prokhorova and Natalya Zhukova and Anna Lemza and Mariia Sergeeva and Rinat Amirkhanov and Grigory Stepanov",
note = "Funding Information: Funding: This research was supported within the framework of the state assignment of the ICBFM SB RAS [number 0245-2022-0002] and was supported by the RFBR [grant number 18-29-07073]. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = feb,
doi = "10.3390/v14020437",
language = "English",
volume = "14",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",
}