Amplified fragments of an autosome-borne gene constitute a significant component of the w sex chromosome of eremias velox (Reptilia, lacertidae)

Artem Lisachov, Daria Andreyushkova, Guzel Davletshina, Dmitry Prokopov, Svetlana Romanenko, Svetlana Galkina, Alsu Saifitdinova, Evgeniy Simonov, Pavel Borodin, Vladimir Trifonov

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

Аннотация

Heteromorphic W and Y sex chromosomes often experience gene loss and heterochromatinization, which is frequently viewed as their “degeneration”. However, the evolutionary trajectories of the heterochromosomes are in fact more complex since they may not only lose but also acquire new sequences. Previously, we found that the heterochromatic W chromosome of a lizard Eremias velox (Lacertidae) is decondensed and thus transcriptionally active during the lampbrush stage. To determine possible sources of this transcription, we sequenced DNA from a microdissected W chromosome sample and a total female DNA sample and analyzed the results of reference-based and de novo assembly. We found a new repetitive sequence, consisting of fragments of an autosomal protein-coding gene ATF7IP2, several SINE elements, and sequences of unknown origin. This repetitive element is distributed across the whole length of the W chromosome, except the centromeric region. Since it retained only 3 out of 10 original ATF7IP2 exons, it remains unclear whether it is able to produce a protein product. Subsequent studies are required to test the presence of this element in other species of Lacertidae and possible functionality. Our results provide further evidence for the view of W and Y chromosomes as not just “degraded” copies of Z and X chromosomes but independent genomic segments in which novel genetic elements may arise.

Язык оригиналаанглийский
Номер статьи779
Страницы (с-по)NA
ЖурналGenes
Том12
Номер выпуска5
DOI
СостояниеОпубликовано - 20 мая 2021

Предметные области OECD FOS+WOS

  • 3.02 КЛИНИЧЕСКАЯ МЕДИЦИНА
  • 1.06 БИОЛОГИЧЕСКИЕ НАУКИ
  • 1.06.KM ГЕНЕТИКА И НАСЛЕДСТВЕННОСТЬ

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