A Platform for Studying Neurodegeneration Mechanisms Using Genetically Encoded Biosensors

E. I. Ustyantseva, S. P. Medvedev, A. S. Vetchinova, J. M. Minina, S. N. Illarioshkin, S. M. Zakian

Результат исследования: Научные публикации в периодических изданияхстатьярецензирование

3 Цитирования (Scopus)


Patient-specific induced pluripotent stem cells (iPSCs) capable of differentiation into required cell type are a promising model for studying various pathological processes and development of new therapeutic approaches. However, no conventional strategies for using iPSCs in disease research have been established yet. Genetically encoded biosensors can be used for monitoring messenger molecules, metabolites, and enzyme activity in real time with the following conversion of the registered signals in quantitative data, thus allowing evaluation of the impact of certain molecules on pathology development. In this article, we describe the development of a universal cell-based platform for studying pathological processes associated with amyotrophic lateral sclerosis. For this purpose, we have created a series of plasmid constructs for monitoring endoplasmic reticulum stress, oxidative stress, apoptosis, and Ca 2+ -dependent hyperexcitability and generated transgenic iPSC line carrying mutation in the superoxide dismutase 1 gene (SOD1) and healthy control cell line. Both cell lines have specific transactivator sequence required for doxycycline-controlled transcriptional activation and can be used for a single-step biosensor insertion.

Язык оригиналаанглийский
Страницы (с-по)299-309
Число страниц11
ЖурналBiochemistry (Moscow)
Номер выпуска3
СостояниеОпубликовано - 1 мар. 2019


Подробные сведения о темах исследования «A Platform for Studying Neurodegeneration Mechanisms Using Genetically Encoded Biosensors». Вместе они формируют уникальный семантический отпечаток (fingerprint).